| 高丹草新品系的SSR分析 |
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| 引用本文： | 李晓宇,于肖夏,马艳红,于卓,房永雨. 高丹草新品系的SSR分析[J]. 中国草地学报, 2011, 33(5) |
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| 作者姓名： | 李晓宇 于肖夏 马艳红 于卓 房永雨 |
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| 作者单位： | 内蒙古农业大学农学院,内蒙古 呼和浩特,010019 |
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| 基金项目： | 国家重点基础研究发展计划(“973”计划)项目“中国西部牧草、乡土草遗传及选育的基础研究”(2007CB108903); 国家自然科学基金项目(31060322); 内蒙古农业综合开发办项目(2010-97); 内蒙古自然科学基金重大项目(200711020301) |
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| 摘 要： | 对11份高丹草新品系(品种)进行了SSR多态性分析.用筛选出的9对SSR适宜引物对高丹草各材料基因组DNA进行PCR扩增,得到318个多态性位点,多态性位点比率达95.78％.引物Xtxp13及Xtxp67扩增的SSR指纹图谱差异明显,可作为11个高丹草新品系(品种)鉴别的分子依据.11份高丹草材料间的GD值变动在0.4286～0.7226之间,以GD值0.61为基准,可将11份材料分为3类:第一类包括SLCN01、SLCN02、SLCN03、SLCN09、SLCN10和蒙农青饲3号高丹草；第二类包括SLCN04、SLCN05、SLCN06、SLCN08；SLCN07单独为一类.
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| 关 键 词： | 高丹草 新品系 遗传差异 SSR分析 |
SSR Analysis of New Strains of Sorghum-Sudangrass |
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| LI Xiao-yu,YU Xiao-xia,MA Yan-hong,YU Zhuo,FANG Yong-yu. SSR Analysis of New Strains of Sorghum-Sudangrass[J]. Chinese Journal of Grassland, 2011, 33(5) |
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| Authors: | LI Xiao-yu YU Xiao-xia MA Yan-hong YU Zhuo FANG Yong-yu |
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| Affiliation: | LI Xiao-yu,YU Xiao-xia,MA Yan-hong,YU Zhuo,FANG Yong-yu(Agronomy College,Inner Mongolia Agricultural University,Hohhot 010019,China) |
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| Abstract: | SSR polymorphism of 11 new strains of Sorghum-Sudangrass was analyzed.Genomic DNAs of Sorghum-Sudangrass were amplified with nine pairs of SSR primers,there were 318 polymorphic loci,the percentage of polymorphic loci reached 95.78%.There were significant differences between fingerprint map amplified with primer Xtxp13 and fingerprint map amplified with primer Xtxp67,which can be used as molecular basis for identifying the eleven new strains(cultivars) of Sorghum-Sudangrass.Genetic distance(GD) among the ne... |
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| Keywords: | Sorghum New strain Genetic difference SSR analysis |
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