Agarose gel electrophoretic separation of blood serum proteins in cattle. |
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Authors: | Gunnar Carlströ m,Per Liberg |
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Affiliation: | Research Station of the Veterinary Institute, S-532 00 Box 180, Skara, Sweden |
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Abstract: | The agarose gel electrophoresis described by Johansson (1972) was modified so that a buffer of pH 7.9 was used in the gel, whereas the buffer in the electrode vessels had a pH of 8.6.The cattle blood serum protein picture is described in detail. The β1-globulin zone shows a very distinct picture of the genetically polymorphic bovine transferrins. The region between the α- and β-globulins shows a number of faint and often very distinct bands. A faint background staining over the whole electrophoretogram may partly be caused by a rather strong lipoprotein in the α1-region, lipids thus having migrated all over the electrophoretogram.The modified method described is well suited as a “screen electrophoresis” for cattle serum and is also useful e.g. in studying bovine transferrin polymorphism. |
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Keywords: | electrophoresis agarose gel electrophoresis screen bovine serum cattle |
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