| 香蕉NBS-LRR抗病基因类似序列的克隆和分析 |
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| 引用本文： | 莫庭辉,黄俊生,杜中军,徐立,陈业渊.香蕉NBS-LRR抗病基因类似序列的克隆和分析[J].热带作物学报,2006,27(3):46-50. |
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| 作者姓名： | 莫庭辉 黄俊生 杜中军 徐立 陈业渊 |
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| 作者单位： | 华南热带农业大学农学院,海南,儋州,571737;中国热带农业科学院热带作物生物技术国家重点实验室,海口,571101;中国热带农业科学院热带作物生物技术国家重点实验室,海口,571101;中国热带农业科学院热带品种资源研究所,海南,儋州,571737;中国热带农业科学院热带品种资源研究所,海南,儋州,571737 |
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| 基金项目： | This work was supported by China Postdoctoral Science Foundation (Contact No. 2005037628) and Science and Technology Foun-dation of South China Agricultural University (Contact No. Rnd0401) |
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| 摘 要： | 根据大多数抗病基因的核苷酸结合区(NBS)和富含亮氨酸重复序列(LRR)保守区的氨基酸序列设计PCR简并引物,利用快速而又可行的简并PCR技术获得NBS-LRR类抗病基因类似序列。笔者利用该技术从香蕉cDNA中获得了一个NBS-LRR类抗病基因类似序列,命名为B-NB(SAY739270)。结构域分析发现,B-NBS含有NBS蛋白所具有的P-Loop、Kinase-2、Kinase-3a和下游疏水GLPLAL结构域(HD,GLPLAL)4个保守基序。聚类分析发现,B-NBS与已报道的6个植物抗病基因的NBS基序的遗传距离在21.7之内。因此,B-NBS是一个香蕉NBS-LRR类抗病基因类似序列。本研究将为香蕉抗病基因克隆和抗病机理研究提供新的抗病候选基因资源。
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| 关 键 词： | 香蕉 抗病基因 类似序列 NBS-LRR |
| 收稿时间： | 2005-12-29 |
| 修稿时间： | 2005年12月29 |
Cloning and Characterization of One NBS-LRR like Resistance Gene Analog from Banana |
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| Mo Tinghui,Huang Junsheng,Du Zhongjun,Xu Li,Chen Yeyuan.Cloning and Characterization of One NBS-LRR like Resistance Gene Analog from Banana[J].Chinese Journal of Tropical Crops,2006,27(3):46-50. |
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| Authors: | Mo Tinghui Huang Junsheng Du Zhongjun Xu Li Chen Yeyuan |
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| Institution: | 1.College of South China University of Tropical Agriculutal Danzhou Hainan 571737 China ;2 .State Key Biotechnology Laboratory for Tropical Crops, Chinese Academy of Tropical Agricultural Sciences Haikou Hainan 571101 China ;3 .Tropical Crops Genetic Resources institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou Hainan 571737 China |
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| Abstract: | Degenerated PCR using primers targeted to NBS conserved motifs is a rapid and facile strategy for isolation of NBS sequences linked to disease resistance genes.This PCR-based approach was used isolate and characterize a NBS-LRR like resistance gene analog (GenBank Accession No.AY739270) from banana, which was named B-NBS. After the multialignment with NBS domains of other six known plant R proteins, four NBS conserved domains were found in B-NBS, which were 'P-Loop', 'Kinase-2', 'Kinase-3a' and 'Hydrophobic domain'. By clustering analysis, all genetic distance of B-NBS with NBS domains of other six known plant R proteins was found smaller than 21.7. This showed that B-NBS was an NBS-LRR like resistance gene analog (RGA). This will lay a RGAs resources foundation for cloning banana R-gene and studying its disease resistance mechanism. |
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| Keywords: | banana R-gene analog NBS-LRR |
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