| 辣椒青枯病菌的分离及其PCR检测 |
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| 引用本文： | 罗香文,程菊娥,成飞雪,朱春晖,刘勇.辣椒青枯病菌的分离及其PCR检测[J].湖南农业科学,2007(4):130-131,134. |
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| 作者姓名： | 罗香文 程菊娥 成飞雪 朱春晖 刘勇 |
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| 作者单位： | 1. 湖南农业大学,湖南,长沙,410128
2. 湖南省植物保护研究所,湖南,长沙,410126 |
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| 基金项目： | 国家“十一五”科技支撑计划项目(2006BAD17B08) |
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| 摘 要： | 从辣椒上分离的青枯病菌用TTC、523平板划线培养可得到典型的单菌落(中央粉红色或红色,具流动性),共分离到20个纯培养物。实验不需要提取青枯病菌的DNA,可直接利用细菌粗悬液进行PCR扩增反应,并克隆到281 bp的分子片段。实验结果为鉴定辣椒青枯病菌株提供了一种快速特异的检测手段。
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| 关 键 词： | 青枯病菌 分离 PCR |
| 文章编号： | 1006-060X（2007）04-0130-02 |
| 修稿时间： | 2007-05-28 |
The Isolation and Specific Detection by PCR of Ralstonia Solanacearum in Pepper |
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| LUO Xiang-wen,CHENG Ju-e,CHENG Fei-xue,ZHU Chun-hui,LIU Yong.The Isolation and Specific Detection by PCR of Ralstonia Solanacearum in Pepper[J].Hunan Agricultural Sciences,2007(4):130-131,134. |
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| Authors: | LUO Xiang-wen CHENG Ju-e CHENG Fei-xue ZHU Chun-hui LIU Yong |
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| Institution: | 1.Hunan Agricultural University,Changsha 410128,PRC;2.Hunan Plant Protection Institute,Changsha 410125,PRC |
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| Abstract: | The typical single colonies(fluidal with pink or red center) were obtained from different R.solanacearum strains isolated from pepper streaking on TTC and 523 plates,and then 20 purified cultures were isolated.A specific amplification fragment of 281bp was obtained for bacterium using the primer 759/760.The results provided a fast means of detection for specific identifying strains of R.solanacearum in pepper. |
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| Keywords: | R solanacearum of pepper isolate PCR |
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