| 苦瓜花粉体外萌发培养基成分的优化 |
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| 引用本文： | 胡新军,粟建文,袁祖华,李勇奇,刘志敏.苦瓜花粉体外萌发培养基成分的优化[J].湖南农业大学学报(自然科学版),2009,35(1). |
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| 作者姓名： | 胡新军 粟建文 袁祖华 李勇奇 刘志敏 |
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| 作者单位： | 1. 湖南省农业科学院,蔬菜研究所,湖南,长沙,410125
2. 湖南农业大学,园艺园林学院,湖南,长沙,410128 |
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| 基金项目： | 农业部公益性行业科研专项 |
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| 摘 要： | 应用二次正交旋转通用组合设计,研究了培养基中蔗糖、Ca2+和硼酸3浓度对苦瓜花粉体外萌发的影响,获得了相应的数学模型,解析了各因素对苦瓜花粉萌发率的主效应和互作效应,通过模型寻优获得了适于苦瓜花粉体外萌发的最优培养基配方.结果表明,3种成分对苦瓜花粉萌发率的影响从大到小依次为蔗糖、硼酸、Ca2+.在本试验条件下,苦瓜花粉萌发率大于73%的培养基配方为:蔗糖质量浓度8.0%～8.8%,硼酸浓度1.59～1.84 mmol/L,Ca2+浓度2.98～3.44 mmol/L.
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| 关 键 词： | 苦瓜 花粉萌发 培养基 |
Optimization of composition of culture mediums for in vitro germination of bitter gourd pollen |
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| HU Xin-jun,SU Jian-wen,YUAN Zu-hua,LI Yong-qi,LIU Zhi-min.Optimization of composition of culture mediums for in vitro germination of bitter gourd pollen[J].Journal of Hunan Agricultural University,2009,35(1). |
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| Authors: | HU Xin-jun SU Jian-wen YUAN Zu-hua LI Yong-qi LIU Zhi-min |
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| Institution: | 1.Institute of Vegetable;Hunan Academy of Agricultural Sciences;Changsha 410125;China;2.College of Horticulture and Landscape;HNAU;Changsha 410128;China |
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| Abstract: | The effect of the concentration of sucrose, boric acid and Ca2+ in the cultural media on in vitro germination for bitter melon pollen was investigated, and a mathematic model was established involving the three factors and the germination rate by using the quadric orthogonal rotation general combination design. The major and interactive effect of the three factors on the germination rate were determined, and the optimum formulation of the cultural media for in vitro germination of bitter melon pollen was fo... |
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| Keywords: | Momordica charantia L pollen germination in vitro medium |
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