甘肃省河西地区辣(甜)椒病毒病毒原鉴定

2006～2009年用DAS-ELISA方法对采自甘肃省河西地区辣(甜)椒病毒病病株的27份样本进行了烟草花叶病毒(TMV)、黄瓜花叶病毒(CMV)、烟草蚀纹病毒(TEV)、辣椒轻微斑驳病毒(PMMoV)、番茄斑萎病毒(TSWV)、马铃薯X病毒(PVX)、马铃薯Y病毒(PVY)、苜蓿花叶病毒(AMV)和蚕豆萎蔫病毒(BBWV)共9种病毒的检测,同时采用反转录聚合酶链式反应对其中20份样本进行了前4种病毒的测定。结果表明,DAS-ELISA方法检测出的病毒种类有TMV、CMV、PMMoV、PVY和TEV,其中TMV和CMV是优势毒原种群,检出率分别为44.4%和33.3%;RT-PCR方法检测出TMV、CMV、TEV和PMMoV;未检出TSWV、PVX、AMV和BBWV。

Identification of Viruses Infecting Capsicum annuum L.in Hexi Area of Gansu Province

In a survey during 2006-2009, 27 pepper（Capsicum anmuum L.）samples with viral symptoms collected from the Hexi Area of Gansu Province were detected by DAS-ELISA with antibodies against nine plant viruses. The results showed that these samples were infected by the viruses of Tobacco mosaic virus（TMV）, Cucumber mosaic virus（CMV）, Pepper mild mottle virus（PMMoV）, Potato virus Y（PVY）and Tobacco etch virus（TEV）. TMV and CMV were the major viruses infecting pepper in Hexi Area with relevance ratio of 44.4 % and 33.3 %, respectively. Moreover, TMV, CMV, TEV and PMMoV in 20 samples among the 27 samples were tested by RT-PCR. The results indicated that these samples were infected by TMV, CMV, TEV and PMMoV. Tests for Tomato spotted wilt virus（TSWV）, Potato virus X（PVX）, Alfalfa mosaic virus（AMV）and Broad bean wilt virus（BBWV）showed that these viruses were not present in these collected pepper samples.