| GUS基因瞬时表达检测香菇顺式因子的调控功能 |
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| 引用本文： | 胡乐琴,姚泉洪,陈明杰,熊爱生,潘迎捷. GUS基因瞬时表达检测香菇顺式因子的调控功能[J]. 上海水产大学学报, 2006, 15(3): 276-280 |
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| 作者姓名： | 胡乐琴 姚泉洪 陈明杰 熊爱生 潘迎捷 |
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| 作者单位： | 上海水产大学生命科学与技术学院 上海200090(胡乐琴)，上海农业科学研究院生物中心 上海201106(姚泉洪，熊爱生)，上海农业科学研究院食用菌研究所 上海201106(陈明杰)，上海水产大学食品学院 上海200090(潘迎捷) |
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| 基金项目： | 上海市科技兴农科技攻关项目 |
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| 摘 要： | 设计了一个顺式元件功能检测载体pYF3553,该载体以GUS为报告基因,其上游连接一段由待测香菇(Lentinula edodes)克隆片段与酵母Cyc1基本启动子组成的杂合启动子。将编号为XG108和XG111的香菇克隆序列构建的检测载体命名为pXG108p、XG111。将pXG108转化到香菇原生质体中,结果被测样的GUS瞬时活性比对照增加一倍,表明GUS基因得到了表达,其上游调控元件具有调控功能。对比pXG108和pXG111转化后的表达结果,其GUS瞬时表达活性表现出差异,这种差异与克隆片段在酵母中的表达差异相一致。分析了利用GUS瞬时表达验证未知香菇顺式元件的优缺点。
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| 关 键 词： | 香菇 顺式调控元件 转化 GUS基因 瞬时表达 |
| 文章编号： | 1004-7271（2006）03-0276-05 |
| 收稿时间： | 2006-03-29 |
| 修稿时间： | 2006-03-29 |
Checking of cis-acting element of Lentinula edodes by transient expression of GUS gene |
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| HU Le-qin,YAO Quan-hong,CHEN Ming-jie,XIONG Ai-sheng,PAN Ying-jie. Checking of cis-acting element of Lentinula edodes by transient expression of GUS gene[J]. Journal of Shanghai Fisheries University, 2006, 15(3): 276-280 |
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| Authors: | HU Le-qin YAO Quan-hong CHEN Ming-jie XIONG Ai-sheng PAN Ying-jie |
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| Affiliation: | 1. College of Aqua-life Science and Technology, Shanghai Fisheries University, Shanghai 200090, China ; 2. Biology Science Central lab, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 3. Edible Fungi Institute, Shanghai Acodemy of Agricultural Sciences, Shanghai 201106, China ; 4. College of Food Science, Shanghai Fisheries University, Shanghai 200090, China |
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| Abstract: | A function-checking vector,pYF3553 was constructed,GUS gene as the reporter gene,whose upstream was the fusion-promoter consisted of cis-acting elements of Lentinula edodes and a yeast Cyc1 gene mini-promoter.Two function-checking vector,whose cis-acting elements of Lentinula edodes were XG108 and XG111,were named pXG108 and pXG111.By transforming of the pXG108 into protoplasts of Lentinula edodes,transient expression of GUS activity was twice as high as the control.This phenomenon revealed the obvious expression of GUS gene and the modulation function of its upstream sequence.Transient GUS gene expression of the two vector,pXG108 and pXG111,was different.Difference existed in the transient GUS gene expression between the two cis-acting elements of Lentinula edodes,pXG108 and pXG111 resemble the expression effect in yeast of that.A detailed analysis of superiority and disadvantage for the method utilizing the transient GUS gene expression to detect the unknow function of cis-acting elements of edible fungi was also brought forward. |
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| Keywords: | Lentinula edodes cis-acting element GUS gene transient expression |
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