猪附红细胞体病BALB/c小鼠模型的建立

为了解现阶段延边地区猪附红细胞体的致病性,以注射地塞米松和摘除脾脏的BALB/c小鼠为实验动物,以腹腔注射方式人工感染猪附红细胞体,通过临床症状观察、血液涂片姬姆萨染色镜检及PCR方法对BALB/c小鼠感染猪附红细胞体情况进行鉴定,并进行血液常规检测。结果表明,注射地塞米松组、摘除脾脏组和正常感染组BALB/c小鼠在感染后8～10d相继达到高峰,正常感染组BALB/c小鼠在感染后第24天猪附红细胞体消失,注射地塞米松组在感染后第32天消失,而摘除脾脏组在试验结束时仍具有较高的感染率。经血液常规检测,各组BALB/c小鼠在感染猪附红细胞体后,白细胞总数显著升高(P<0.01),淋巴细胞总数、红细胞总数、血红蛋白含量均显著下降(P<0.05),红细胞比容显著下降(P<0.01)。因此,猪附红细胞体对低免疫力的BALB/c小鼠具有一定感染性,并表现出典型临床症状。

Establishment of BALB/c mice model of swine Eperythrozoonosis

In order to understand the pathogenic of Eperythrozoon suis in Yanbian area,BALB/c mice were divided into 3 groups: BALB/c mice in group 1 were injected with dexamethasone,BALB/c mice’s spleens in group 2 were removed and BALB/c mice in group 3 were normal.The BALB/c mice were artificially infected with E.suis by intraperitoneal injection.BALB / c mice infected with E.suis were identified by clinical observation of symptoms,blood smear,Giemsa staining and PCR method.And routine blood test was carried out.The results showed that the infection rates of the 3 groups reached their peaks in 8~10 days after infection.E.suis was disappeared in group 3 in the 24 th day after infection.E.suis was disappeared in group 1 in the 32 nd day after infection.However,the infection rates of the group 2 was still high.Routine blood test showed that after infected with E.suis in each group,the total number of white blood cells was significantly increased(P<0.01),the total number of lymphocyte,red blood cells and the level of hemoglobin were significantly decreased(P<0.05).Red blood cells and the hematocrit was significantly decreased(P<0.01).This test confirmed that BALB/c mice could be infected by E.suis and the infected mice showed typical clinical symptoms.