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Using a linkage mapping approach to identify QTL for day-neutrality in the octoploid strawberry
Authors:C K Weebadde    D Wang    C E Finn    K S Lewers    J J Luby    J Bushakra    T M Sjulin  and J F Hancock
Institution:Michigan State University, East Lansing, Michigan 48824, E-mail:;;ARS-USDA Horticultural Crops Research Laboratory, 3420 NW Orchard Ave., Corvallis, Oregon 97330;;USDA-ARS, Fruit Lab, R 210, 10300 Baltimore Ave., Building 010A BARC- West, Beltsville, Maryland 20705-2350;;R342, Alderman Hall, Department of Horticultural Science, University of Minnesota, 1970 Folwell Ave., St Paul, Minnesota 55108;;Driscoll Strawberry Associates Inc., 404 San Juan Road, Watsonville, California 95077, USA
Abstract:A linkage mapping approach was used to identify quantitative trait loci (QTL) associated with day-neutrality in the commercial strawberry, Fragaria  ×  ananassa (Duch ex Rozier). Amplified Fragment Length Polymorphism (AFLP) markers were used to build a genetic map with a population of 127 lines developed by crossing the day-neutral (DN) 'Tribute' with the short-day (SD) 'Honeoye'. The population was genotyped with AFLP markers and 429 single dose restriction fragments (SDRF) were placed on a consensus map of 1541 cM with 43 linkage groups. Individuals from the mapping population were observed for their flowering habit throughout the growing season in Michigan (MI), Minnesota (MN), Maryland (MD), Oregon (OR) and California (CA). Eight QTL were found that were either location specific or shared among locations. None of these QTL explained >36% of the phenotypic variation, indicating that the inheritance of day-neutrality is likely a polygenic trait.
Keywords:Fragaria × ananassa  everbearing strawberries  photoperiod insensitivity  AFLPs  QTL mapping
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