Metabolism of lindane in house flies: Metabolic desaturation,dehydrogenation and dehydrochlorination,and conjugation with glutathione |
| |
Authors: | Keiji Tanaka Norio Kurihara Minoru Nakajima |
| |
Institution: | 1. Department of Agricultural Chemistry, Kyoto University, Kyoto, Japan;2. Radioisotope Research Center, Kyoto University, Kyoto, Japan |
| |
Abstract: | In lindane-treated house flies, a cis-dehydrogenated metabolite, , was identified by gas-liquid chromatography and mass spectrometry. The in vitro metabolism study showed that in the presence of NADPH the microsomal fraction of house flies converted lindane to three hexane-soluble metabolites. This conversion was inhibited by piperonyl butoxide, SKF-525A, and carbon monoxide. These metabolites were identified as , (PCCHE) by gas-liquid chromatography. They, as well as lindane, were excellent substrates for the reaction with the postmicrosomal fraction in the presence of glutathione. While the reaction with lindane-d6 showed a significant deuterium isotope effect (6.82), that of did not (1.18). Enzymatic conjugation with glutathione probably occurs at the stage of PCCHE. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|