Development and application of G1-ELISA for detection of antibodies against bovine ephemeral fever virus |
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| Authors: | Fu-ying Zheng Guo-zhen Lin Chang-qing Qiu Ji-zhang Zhou Xiao-an Cao Xiao-wei Gong |
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| Institution: | aKey Laboratory of Grazing Animal Diseases of Ministry of Agriculture, Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China |
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| Abstract: | The gene encoding antigenic site G1 of bovine ephemeral fever virus (BEFV) was highly expressed in the host cell Escherichia coli. An indirect G1-ELISA with the recombinant protein as the coating antigen was established to detect antibodies against BEFV. The result revealed that the optimal concentration of the coated antigen was 0.5 μg/well and the dilution of serum was 1:20. It was optimal that sera with P/N value  2.2 were considered positive, P/N value  2.0 negative, and between 2.0 and 2.2 ambiguous. The G1-ELISA method gave a sensitivity of 97.6% and a specificity of 98.6% by testing 590 field serum samples. These results suggest that the G1-ELISA may be a good alternative tool for seroepidemiological surveys. |
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| Keywords: | Bovine ephemeral fever virus Indirect ELISA MNT Recombinant protein |
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