| 液体菌种栽培毛木耳研究 |
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| 引用本文： | 韦珂,;江民华,;覃培升,;莫琪红,;蒙健宗.液体菌种栽培毛木耳研究[J].广西园艺,2014(4):10-12. |
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| 作者姓名： | 韦珂 ;江民华 ;覃培升 ;莫琪红 ;蒙健宗 |
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| 作者单位： | [1]广西大学农学院,广西南宁530005; [2]广西大学生命科学与技术学院,广西南宁530005; |
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| 基金项目： | 广西创新计划（2013CXJHB01）;广西科学研究与技术开发计划（桂科能1346007-26）. |
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| 摘 要： | 采用摇瓶培养方法对毛木耳液体菌种的碳源和氮源进行筛选。结果表明,玉米淀粉和蛋白胨分别为较优的碳源和氮源;通过测定液体培养时菌丝生物量、菌丝球数量的变化以及回管萌发试验确定最适发酵时间为5 d;采用以上条件制备的液体菌种栽培毛木耳,菌丝满袋时间较固体菌种减少6 d,生物学效率与固体接种相近。
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| 关 键 词： | 毛木耳 液体菌种 碳源 氮源 |
Study on Liquid Strain of Auricularia polytricha |
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| Institution: | WEI Ke,JIANG Min-hua,QIN Pei-sheng,MO Qi-hong,MENG Jian-zong ( 1 .College of Agrieuhure, Guangxi University, 2.College of Life Science and Technology, Guangxi University, Nanning, 530004, China) |
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| Abstract: | Through the single factor experiment, the formula of liquid strain of Auricularia polytricha was been optimized by shake flask in order to obtain carbon source and nitrogen source. The results showed that corn starch and the peptone are the best active carbon source and nitrogen source respectively. The optimal incubation time had been determined for 5 day by measuring the growth curve of liquid culture and tie-back test. The growth time of mycelia of liquid strain of Auricularia polytricha cultivated in the optimal condition is less than 6 days comparing to solid strain, and the efficiencies of biological of both are almost equal. |
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| Keywords: | Auricularia polytricha liquid strain carbon source nitrogen source |
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