A comparison of inoculation techniques for inducing aflatoxin contamination and<Emphasis Type="Italic">Aspergillus flavus</Emphasis> kernel infection on corn hybrids in the field |
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Authors: | G L Windham W P Williams |
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Institution: | (1) USDA-ARS Corn Host Plant Resistance Research Unit, 39762 Mississippi State, MS, USA |
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Abstract: | Aspergillus flavus inoculation techniques were compared on aflatoxin-resistant and -susceptible corn hybrids for inducing aflatoxin contamination
andA. flavus kernel infection. A dry carrier technique was comparable to the standard inoculation techniques (the side-needle and a spray
technique) in differentiating between the resistant and the susceptible hybrids in the first year of the study. However, only
hybrids inoculated with the side-needle technique had statistically different levels of aflatoxin andA. flavus kernel infection in the second year of the study. In a second study, a modified pinbar technique with inoculations near the
tip or base of the ear was compared with the side-needle technique. When developing ears were inoculated near the base with
the modified pinbar, adequate levels of aflatoxin were induced both years to distinguish between the resistant and susceptible
corn hybrids. The modified pinbar technique has the potential of being a useful tool in evaluating corn germplasm for aflatoxin
resistance.
http://www.phytoparasitica.org posting May 4, 2007. |
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Keywords: | Aflatoxin Aspergillus flavus corn inoculation techniques kernel infection maize |
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