| 牛BMP-15基因cDNA的克隆和序列分析 |
| |
| 引用本文： | 祁云霞,王峰,刘永斌,田蕾,董文杰,达赖,田春英,荣威恒.牛BMP-15基因cDNA的克隆和序列分析[J].华北农学报,2007,22(4):63-66. |
| |
| 作者姓名： | 祁云霞 王峰 刘永斌 田蕾 董文杰 达赖 田春英 荣威恒 |
| |
| 作者单位： | 1. 内蒙古农业大学,动物科学与医学学院,内蒙古,呼和浩特,010018;内蒙古农牧业科学院,内蒙古,呼和浩特,010030
2. 内蒙古农牧业科学院,内蒙古,呼和浩特,010030 |
| |
| 基金项目： | 内蒙古科技攻关项目(20040102)，内蒙古自然科学基金资助项目(200607010410) |
| |
| 摘 要： | BMP-15基因主要在哺乳动物卵巢中表达,对卵泡的发育和分化起重要作用,克隆了牛BMP-15成熟肽编码区的cDNA序列并进行序列分析,旨在为BMP-15在牛繁殖性能方面的进一步研究奠定理论基础。根据其他物种BMP-15基因的保守序列设计特异性引物,扩增牛的cDNA序列。从牛卵巢中提取总RNA,采用RT-PCR技术扩增出牛BMP-15cDNA序列。将此片段克隆到PMD18-T载体中,经菌落PCR鉴定和DNA序列测定分析验证,证实所克隆序列BMP-15为骨形态发生蛋白,符合骨形态发生蛋白基因的特征。序列分析表明,该cDNA包含有1 185 bp组成的开放读码框(ORF),该ORF编码394个氨基酸,与绵羊、猪、人、小鼠等动物氨基酸序列比对发现同源性分别为98.5%,87.6%,74.0%,69.4%。
|
| 关 键 词： | 牛 BMP-15 RT-PCR 克隆 序列分析 |
| 文章编号： | 1000-7091（2007）04-0063-04 |
| 修稿时间： | 2007-03-21 |
The Clone and Sequence of Bovine BMP-15 Gene cDNA |
| |
| QI Yun-xia,WANG Feng,LIU Yong-bin,TIAN Lei,DONG Wen-jie,DA Lai,TIAN Chun-ying,RONG Wei-heng.The Clone and Sequence of Bovine BMP-15 Gene cDNA[J].Acta Agriculturae Boreali-Sinica,2007,22(4):63-66. |
| |
| Authors: | QI Yun-xia WANG Feng LIU Yong-bin TIAN Lei DONG Wen-jie DA Lai TIAN Chun-ying RONG Wei-heng |
| |
| Institution: | 1. College of Animals Science and Medicine, Inner Mongolia Agriculture University, Huhhot 010018, China; 2. Inner Mongolia Academy of Agriculture and Husbundary Sciences, Huhhot 010030, China |
| |
| Abstract: | BMP-15(Bone Morphogenetic Protein-15)gene has important role in the development and differentiation of follicle and is specialized expressed in mammalian ovaries.In order to lay the theoretical foundations for further study on BMP-15 in bovine reproduction,cDNA sequence of bovine BMP-15 was cloned and analyzed.The specific primers according to conserver sequence of the other animals was designed to amplify bovine BMP-15 cDNA with RT-PCR The purified RT-PCR product was cloned into PMD18-T vector.The results showed that the bovine BMP-15 belonged to the family of bone morphogenetic protein because of an opening reading frame(ORF).The ORF contained 1 185 base pairs which encoded 394 amino acid pre-pro-peptide and the pre-pro-peptide contained the BMP consensus sequence of six invariantly spaced cysteines residues.Compared with the sequence of sheep,pig,human,mouse and the ORF homology were 98.5%,87.6%,74.0%,69.4% respectively. |
| |
| Keywords: | Bovine Bone Morphogenetic Protein-15 RT-PCR Cloning Sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
