| 人工诱变芦荟多倍体的初步研究 |
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| 引用本文： | 任清 李守勉 姚彬 王潇潇. 人工诱变芦荟多倍体的初步研究[J]. 华北农学报, 2007, 22(B10): 136-138 |
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| 作者姓名： | 任清 李守勉 姚彬 王潇潇 |
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| 作者单位： | [1]北京工商大学植物资源研究开发重点实验室,北京100037 [2]河北农业大学园艺学院,河北保定071001 |
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| 摘 要： | 以秋水仙索为诱变剂，利用茎段浸泡和秋水仙素培养基2种方法对库拉索芦荟进行诱变，结果表明，茎段浸泡秋水仙素法诱导四倍体葡萄的效果优于秋水仙素培养基法，0．2％的秋水仙素处理4d变异效果最好，诱变率达到最高值41.5％。变异株叶片肥厚，叶色深绿，叶片表皮气孔保卫细胞中叶绿体数增多。
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| 关 键 词： | 芦荟 秋水仙素 多倍体 |
| 文章编号： | 1000-7091（2007）增刊-0136-03 |
| 修稿时间： | 2007-03-25 |
Studies on Induction of Autotetraploid of Aloe vera L. |
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| REN Qing, LI Shou-mian, YA Bin, WANG Xiao-xiao. Studies on Induction of Autotetraploid of Aloe vera L.[J]. Acta Agriculturae Boreali-Sinica, 2007, 22(B10): 136-138 |
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| Authors: | REN Qing LI Shou-mian YA Bin WANG Xiao-xiao |
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| Affiliation: | 1. Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University, Beijing 100037, China;2. College of Horticulture, Agricultural University of Hebei, Baoding 071001, China |
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| Abstract: | Tetraploids were induced from in vitro Aloe vera L by soaking shoot segments in colchicine solution and adding colchicine in the culture medium. The results showed that the adding colchicine in the culture medium increased the efficiency of induced tetraploids than one of soaking treatment. The optimal conditions for inducing tetraploids were adding colchicine in the culture medium for 3 to 4 d with colchicine at a concentration of 2 000 mg/L. The rate of induction was 41.5 %. Compared with the diploids,the appearance of variations is distinct including plumper leaves and more chloroplasts in stomatal guard cells. |
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| Keywords: | Aloe vera L Colchicines Muhiploid |
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