| 家蚕基因组DNA甲基化分析 |
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| 引用本文： | 翁宏飚,曹锦如,叶爱红,牛宝龙,何丽华,沈卫锋,孟智启. 家蚕基因组DNA甲基化分析[J]. 蚕业科学, 2008, 34(1): 28-32 |
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| 作者姓名： | 翁宏飚 曹锦如 叶爱红 牛宝龙 何丽华 沈卫锋 孟智启 |
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| 作者单位： | 浙江省农业科学院蚕桑研究所,杭州,310021;浙江省农业科学院蚕桑研究所,杭州,310021;浙江省农业科学院蚕桑研究所,杭州,310021;浙江省农业科学院蚕桑研究所,杭州,310021;浙江省农业科学院蚕桑研究所,杭州,310021;浙江省农业科学院蚕桑研究所,杭州,310021;浙江省农业科学院蚕桑研究所,杭州,310021 |
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| 基金项目： | 浙江省科技计划，浙江省自然科学基金 |
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| 摘 要： | DNA甲基化在生物的基因表达调控与发育调节过程中具有重要作用。应用甲基化敏感扩增多态性(MSAP)技术对7个家蚕保存品种的胞嘧啶甲基化模式和程度进行评估。在所能检测的CCGG序列中,至少有8.6%~10.9%的胞嘧啶发生了甲基化;12对引物共获得1 156条扩增带,其中376条在品种间呈多态性,多态性比例为32.5%。结果显示:家蚕基因组可检测到DNA甲基化,各品种间的甲基化模式存在差异,CCGG序列中胞嘧啶外部甲基化(10.9%)高于内部甲基化(8.6%)。
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| 关 键 词： | 家蚕 DNA甲基化 甲基化敏感扩增多态性 |
Analysis of DNA Methylation in Silkworm,Bombyx mori Using MSAP Markers |
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| WENG Hong-Biao,CAO Jin-Ru,YE Ai-Hong,NIU Bao-Long,HE Li-Hua,SHEN Wei-Feng,MENG Zhi-Qi. Analysis of DNA Methylation in Silkworm,Bombyx mori Using MSAP Markers[J]. Acta Sericologica Sinica, 2008, 34(1): 28-32 |
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| Authors: | WENG Hong-Biao CAO Jin-Ru YE Ai-Hong NIU Bao-Long HE Li-Hua SHEN Wei-Feng MENG Zhi-Qi |
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| Abstract: | DNA methylation plays an important role in the process of development and gene expression.The extent and pattern of cytosine methylation in 7 silkworm strains were assessed by using the technique of methylation-sensitive amplification polymorphism(MSAP).8.6%～10.9%DNA methylation events were detected in the CCGG sequence of silkworm genomes.Totally 1 156 fragments were amplified with 12 pairs of primers,with 376 DNA methylation polymorphisms.The average proportion of polymorphisms was 32.5%.The results showed that the DNA methylation patterns were different among the silkworm strains.The external cytosine methylation of the CCGG sequence(10.9%) was more frequent than that of internal one(8.6%). |
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| Keywords: | Bombyx mori DNA methylation Methylationsensitive amplification polymorphism |
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