| 利用荧光定量PCR进行饲料中牛羊源性成分检测的快速筛选 |
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| 引用本文： | 姜艳彬,王海,李颖,侯东军,杨红菊,金秀娥,于雷. 利用荧光定量PCR进行饲料中牛羊源性成分检测的快速筛选[J]. 中国畜牧兽医, 2010, 37(6): 36-39 |
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| 作者姓名： | 姜艳彬 王海 李颖 侯东军 杨红菊 金秀娥 于雷 |
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| 作者单位： | （1.中国动物疫病预防控制中心， 北京 100125； 2.湖北兽药监察所， 武汉 430064） |
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| 摘 要： | 通过荧光定量PCR进行饲料中牛羊源性成分的快速筛选研究,其可以快速的检测饲料中是否含有牛羊源性成分,避免了常规检测中大量阴性样品造成的巨大工作量,并首次对饲料中牛羊源性成分进行定量分析,推导出定量计算公式,且灵敏度比常规的PCR检测方法高100倍以上。
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| 关 键 词： | 牛羊源成分 饲料 荧光定量PCR 快速筛选 |
Rapid Screening of Cattle or Sheep-derived Materials in Feed Products with Quantitative Polymerase Chain Reaction |
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| JIANG Yan-bin,WANG Hai,LI Ying,HOU Dong-jun,YANG Hong-ju,JIN Xiu-e,YU Lei. Rapid Screening of Cattle or Sheep-derived Materials in Feed Products with Quantitative Polymerase Chain Reaction[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(6): 36-39 |
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| Authors: | JIANG Yan-bin WANG Hai LI Ying HOU Dong-jun YANG Hong-ju JIN Xiu-e YU Lei |
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| Affiliation: | (1.China Animal Disease Control Center,Beijing 100125,China;2.Hubei Institute of Veterinary Drug Control,Wuhan 430064,China) |
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| Abstract: | A new method that can rapidly screen cattle or sheep-derived materials in feed products was carried out with quantitative polymerase chain reaction, thus can greatly cut the workload and save the time. Also, quantitative analysis of cattle or sheep-derived materials was carried out together with its formula. The sensivity of this method was 100 times more than the regular PCR one’s. |
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| Keywords: | cattle or sheep-derived materials feed product quantitative PCR rapid screening |
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