| 矮牵牛愈伤组织诱导及原生质体的分离与纯化 |
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| 引用本文： | 王梦茹,曾丽,张邀月,彭勇政,陶懿伟.矮牵牛愈伤组织诱导及原生质体的分离与纯化[J].上海交通大学学报(农业科学版),2019(6):195-200. |
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| 作者姓名： | 王梦茹 曾丽 张邀月 彭勇政 陶懿伟 |
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| 作者单位： | 上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240;农业部都市农业南方重点实验室,上海 200240,上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240 |
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| 摘 要： | 研究了影响诱导矮牵牛(Petunia hybrid)形成愈伤组织和分离、纯化其原生质体效率的关键因素。结果表明,矮牵牛无菌苗叶片可在含1.0 mg/L 6-苄氨基腺嘌呤(6-BA)和0.1 mg/L萘乙酸(NAA)的MS培养基上形成愈伤组织,用酶液(2%纤维素酶R-10 + 0.5%果胶酶Y-23 + 0.3%离析酶R-10,0.5 mol/L甘露醇)对继代培养10~15 d的愈伤组织在黑暗条件下静置酶解12 h,原生质体产量和活性分别达到3.4×106个/g 和83%。
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| 关 键 词： | 矮牵牛 愈伤组织 原生质体 分离 |
| 收稿时间： | 2017/8/28 0:00:00 |
Study on callus induction and protoplast isolation of Petunia hybrida |
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| WANG Meng-ru,ZENG Li,ZHANG Yao-yue,PENG Yong-zheng and TAO Yi-wei.Study on callus induction and protoplast isolation of Petunia hybrida[J].Journal of Shanghai Jiaotong University (Agricultural Science),2019(6):195-200. |
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| Authors: | WANG Meng-ru ZENG Li ZHANG Yao-yue PENG Yong-zheng and TAO Yi-wei |
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| Institution: | School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China,School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China;Key Laboratory of Urban AgricultureSouth, Ministry of Agriculture, Shanghai 200240, China,School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China,School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China and School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China |
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| Abstract: | Leaves of sterile Petunia hybrida seedlings were used to study the key factors affecting the efficiencies of callus induction and protoplast isolation.The results showed that the callus could be induced on the MS medium containing 1.0 mg/L 6-benzylaminopurine(6-BA) and 0.1 mg/L 1-naphthlcetic acid(NAA).Calluses subcultured in the same medium for 10-15 days were then hydrolyzed with solution containing 2% cellulase R-10,0.5% pectolyase Y-2,0.3% macerozyme R-10 and 0.5 mol/L mannitol in dark for 12 hours and the yield and viability of protoplasts reached up to 3.4×106/gram and 83%,respectively. |
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| Keywords: | Petunia hybrid callus protoplast isolation |
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