Diversity analysis of <Emphasis Type="Italic">Aegilops</Emphasis> species from Morocco using RAPD markers |
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Authors: | B Belkadi N Assali A Filali-Maltouf O Benlhabib |
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Institution: | (1) Laboratory of Microbiology and Molecular Biology, Faculty of Science, University Mohamed V, Avenue Ibn Battouta, B.P. 1014, Rabat, Morocco;(2) Cellular and Molecular Biotechnology Unit, Department of Ecology, Agronomy and Improvement of Plants, Hassan II Institute of Agronomy and Veterinary (IAV), Rabat, Morocco |
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Abstract: | The diversity of 51 representative populations of the 5 Aegilops species from Moroccan collection was analyzed using 22 RAPD primers. We investigated the associations among these 5 Aegilops species (A. geniculata Roth (UUMM), A. triuncialis L. (UUCC), A. ventricosa Tausch (DDNN), A. peregrina (Hackel) Maire et Weiller (UUMM) and A. neglecta Req. ex Bert. subsp. recta (Zhuk.) K. Hammer (UUMMNN)); some diploid species considered as their ancestors; accessions of some neighboring countries and also accessions of Triticums. A total of 650 polymorphic RAPD fragments were amplified. A dendrogram was constructed using the un-weighed pair group method
arithmetic average (UPGMA) and Jaccard`s similarity coefficient. The UPGMA clustering showed a regrouping to the level species
with high level of the structuration of the diversity at A. geniculata. We confirm as reported by other authors, the proximity of N genome to U genome and C genome to M genome and also the difference
between the genomes M and N. Thus, the phylogeny between the species and the different genomes were retracted. |
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