A reliable gender diagnostic PCR assay for jojoba (<Emphasis Type="Italic">Simmondsia chinensis</Emphasis> (Link) Schneider) |
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Authors: | Ayse Gul Ince Mehmet Karaca A Naci Onus |
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Institution: | (1) Faculty of Agriculture, Department of Horticulture, Akdeniz University, 07059 Antalya, Turkey;(2) Faculty of Agriculture, Department of Field Crops, Akdeniz University, 07059 Antalya, Turkey; |
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Abstract: | Jojoba (Simmondsia chinensis (Link) Schneider) is an obligate cross-pollinated shrub native to the Sonora desert. The most valuable product of the jojoba
seeds is the liquid wax or jojoba oil which is marketed extensively in the cosmetic industry. Differing from the most of the
cultivated crop species, jojoba has slow-growing habit, difficulties in the determination of sex at the early seedling stages,
a male-biased ratio and low seed yield. In jojoba, the development of molecular strategies for the identification of sexes
at early stages has been a priority in plantations and breeding programs. Two previous studies reported two candidate male-specific
jojoba DNA markers. However, present study indicated that these markers were not useful in jojoba sex identification. A reliable
gender diagnostic marker for jojoba is, therefore, needed. In the present study a novel jojoba male-specific touch-down polymerase
chain reaction based DNA marker (JMS900) was reported using a total of 120 individual jojoba plants bulked into 16 samples.
This sex specific DNA marker may have considerable theoretical and practical applications in the establishment of jojoba plantation
and breeding studies. |
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