DDT and pyrethroids: Receptor binding in relation to knockdown resistance (kdr) in the house fly |
| |
Authors: | C.P. Chang F.W. Plapp |
| |
Affiliation: | Department of Entomology, Texas A&M University, College Station, Texas 77843 USA |
| |
Abstract: | The nature of target site or knockdown resistance (kdr) to DDT and pyrethroids was studied by investigating specific binding of [14C] DDT and [14C] cis-permethrin to the previously established membrane receptors from the heads of susceptible (sbo) and resistant (kdr) strains of the house fly, Musca domestica L. In vivo studies showed the heads from sbo flies bound two to three times more DDT than those from kdr flies at all doses tested. Reduced binding was also observed in kdr flies in in vitro [14C] DDT binding assays. Scatchard analysis indicated that kdr flies have the same affinity but fewer receptors per milligram protein in the CNS than sbo flies. Assays with [14C] cis-permethrin also showed binding was much reduced in kdr flies in comparison with sbo flies. Based on these results, the nature of the target site insensitivity of kdr flies may relate to their having a reduced number of receptors for the insecticides. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|