Observations on artificial fertilization of eggs and the embryonic and larval development of milkfish,Chanos chanos (Forskal) |
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Authors: | H Chaudhuri JV Juario Jurgenne H Primavera R Samson R Mateo |
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Institution: | Aquaculture Department, Southeast Asian Fisheries Development Center (SEAFDEC), Tigbauan, P.O. Box 256, Iloilo Philippines |
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Abstract: | Hydrated eggs obtained from a female milkfish, Chanos chanos, were artificially fertilized with the milt collected from a male injected with acetone-dried pituitaries of salmon. The fertilized eggs (1.1–1.25 mm in diameter) developed normally in seawater in basins and petri dishes at a salinity of 30–34‰, and successfully hatched in 25–28.5 hours at a temperature of 26.4–29.9°C. The yolk was completely absorbed in about 2.5 days and during this period many postlarvae died. A few larvae were reared up to 5 days but all died on the 6th day. Attempts were made to feed the postlarvae with freshly hatched trochophore larvae of oysters obtained from eggs artificially fertilized in the laboratory. |
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