| 黄瓜L-半乳糖-1,4-内酯脱氢酶cDNA全长的克隆和遗传转化 |
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| 引用本文： | 苑志明,劳杉杉,秦智伟*,周秀艳.黄瓜L-半乳糖-1,4-内酯脱氢酶cDNA全长的克隆和遗传转化[J].东北农业大学学报,2012(7):100-103. |
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| 作者姓名： | 苑志明 劳杉杉 秦智伟* 周秀艳 |
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| 作者单位： | 东北农业大学园艺学院,哈尔滨150030 |
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| 基金项目： | 国家农业科技成果转化资金项目(2010GB2B200131);黑龙江省高校科技创新团队项目(2009td07);黑龙江省蔬菜生物学重点实验室项目 |
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| 摘 要： | 以黄瓜D08108果实为材料,根据GenBank中登录的甜瓜L-半乳糖-1,4-内酯脱氢酶(GalLDH)的序列设计引物,采用RT-PCR方法扩增出黄瓜GalLDH的cDNA全长,GenBank登录号为 HQ446099.黄瓜GalLDH的cDNA序列全长1880 bp,包含一个长为1773 bp的完整开放阅读框,编码590个氨基酸.其核苷酸序列与已知其他植物核苷酸序列间的同源性达70%以上.共检测得到5棵转基因植株
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| 关 键 词： | 黄瓜 L-半乳糖-1 4-内酯脱氢酶 抗坏血酸 克隆 |
Cloning and genetic transformation of cDNA full-length of L-galactono-1,4-lactone dehydrogenase from Cucumis sativus |
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| YUAN Zhiming,LAO Shanshan,QIN Zhiwei,ZHOU Xiuyan.Cloning and genetic transformation of cDNA full-length of L-galactono-1,4-lactone dehydrogenase from Cucumis sativus[J].Journal of Northeast Agricultural University,2012(7):100-103. |
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| Authors: | YUAN Zhiming LAO Shanshan QIN Zhiwei ZHOU Xiuyan |
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| Institution: | (School of Horticulture,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | Took cucumber D08108 as the experiment material,designed primer according to L-galactono-1,4-lactone dehydrogenase(GalLDH) sequence of the melon in GenBank,amplified cDNA full-length of cucumber GalLDH by RT-PCR,and GenBank accession number was HQ446099.Total 1 880 bp cDNA encoding L-galactono-1,4-lactone dehydrogenase(GalLDH) full-length was cloned from fruit of Cucumis sativus cultivar D08108,which contained an open reading frame of 1 773 bp and encoded a protein of 590 amino acid residues.The homology analysis demonstrated that GalLDH shared high similarities of nucleotides with those in other plants over 71%.Five putative transgenic Cucumis sativus were obtained. |
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| Keywords: | Cucumis sativus L-galactono-1 4-lactone dehydrogenase(GalLDH) ascorbic acid(AsA) cloning |
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