| Molecular Cloning and Characterization of Citrate Synthase Gene in Rice （ Oryza sativa） |
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| 引用本文： | ZHANG Shan-shan MING Feng Lu Qun Guo Bin SHEN Da-leng. Molecular Cloning and Characterization of Citrate Synthase Gene in Rice （ Oryza sativa）[J]. 水稻科学, 2005, 12(4): 233-237 |
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| 作者姓名： | ZHANG Shan-shan MING Feng Lu Qun Guo Bin SHEN Da-leng |
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| 作者单位： | [1]Institute of Genetics, State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433, China [2]Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute oJ Biodiversity Science, School of LifeScience, Fudan University, Shanghai 200433, China [3]School of Life and Environment Science, Shanghai Normal University.Shanghai 200234, China |
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| 基金项目： | ACKN0WLEGEMENTS This work was supported by National Natural Science Foundation of China （No. 30300193） |
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| 摘 要： | The full-length OsCS encoding citrate synthase was isolated from rice （Oryza sativa L. subsp, japonica）, OsCS is 1477-bp long and encodes a 474 amino acid polypeptide, Its putative protein sequence is highly identical to Daucus carota, Nicotiana tabacum Beta vulgaris subsp., Arabidopsis thaliana, and Citrus junos （〉70%）. The deduced amino-terminal sequence of OsCS showes characteristics of mitochondrial targeting signal. Southern blot analysis using ORF of the OsCS as the probe indicated that this gene exists in multiple copies in rice genome. The band with predicated size of 82 kD was detected by Western blot after being induced by 0,4 mmol/L IPTG.
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| 关 键 词： | 水稻 分子克隆 柠檬酸盐 合酶 基因 |
| 收稿时间： | 2005-09-09 |
| 修稿时间： | 2005-11-11 |
Molecular Cloning and Characterization of Citrate Synthase Gene in Rice( Oryza sativa) |
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| ZHANG;Shan-shan;MING;Feng;Lu;Qun;Guo;Bin;SHEN;Da-leng. Molecular Cloning and Characterization of Citrate Synthase Gene in Rice( Oryza sativa)[J]. Rice science, 2005, 12(4): 233-237 |
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| Authors: | ZHANG Shan-shan MING Feng Lu Qun Guo Bin SHEN Da-leng |
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| Affiliation: | 1.Institute of Genetics, State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433, China;2.Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, School of Life Science, Fudan University, Shanghai 200433, China;3. School of Life and Environment Science, Shanghai Normal University, Shanghai 200234, China |
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| Abstract: | The full-length OsCS encoding citrate synthase was isolated from rice (Oryza sativa L. subsp. japonica). OsCS is 1477-bp long and encodes a 474 amino acid polypeptide. Its putative protein sequence is highly identical to Daucus carota, Nicotiana tabacum, Beta vulgaris subsp., Arabidopsis thaliana, and Citrus junos (>70%). The deduced amino-terminal sequence of OsCS showes characteristics of mitochondrial targeting signal. Southern blot analysis using ORF of the OsCS as the probe indicated that this gene exists in multiple copies in rice genome. The band with predicated size of 82 kD was detected by Western blot after being induced by 0.4 mmol/L IPTG. |
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| Keywords: | citrate synthase rice (Oryza sativa) gene clone |
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