| Abstract: | 14 yellow-seeded rapeseed lines (Brassia napus L. ) from different genetic sources were used toanalyze diversity of testa pigments content, oil and protein content, and RAPD markers. The results showedthat the anthocyanin and melanin were the most important pigments in testa and their content were responsiblefor the variation in seed color ranging from orange to black yellow, 14 yellow-seeded lines could be classifiedinto 3 groups., high anthocyanin content group with anthocyanin content over 2.54 mg g-1 DW, the seed colorwas light yellow or orange; low pigments content group with low content of anthocyanin and melanin, the testawas transparent and the seed color was light yellow, greenish yellow or twany; high melanin content groupwith melanin content over 178.4U(A290nm), the testa was black, the seed color was black yellow. Oil eantentchanged from 36.2% to 45.5%, protein content from 21.1% to 27.7%, and the correlation analysis revealedthat the oil content is highly significantly negatively correlated with the protein content. The cluster analysisshowed that the extensive genetic variation existed among 14 yellow-seeded lines by using unweighted pairedgroup method with arithmetic average (UPGMA) based on RAPD markers which were amplified with decamerprimers, the genetic similarity among them ranged from 0.25 to 0. 909, and 14 yellow-seeded lines could putinto 2 clusters corresponding to genome difference. |
