| 濒危植物崖柏遗传多样性的RAPD分析 |
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| 引用本文： | 刘建锋,肖文发.濒危植物崖柏遗传多样性的RAPD分析[J].江西农业大学学报,2008,30(1):68-72. |
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| 作者姓名： | 刘建锋 肖文发 |
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| 作者单位： | 1. 中国林业科学研究院 林业研究所,北京 100091;国家林业局 林木培育重点实验室,北京 100091
2. 中国林业科学研究院 森林生态环境保护研究所,北京,100091 |
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| 基金项目： | 国务院三峡水力建设委员会、国家林业局“三峡库区陆生野生动植物监测”系统(SX2001-012)，中国林科院科学技术发展研究基金(2004-M-10) |
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| 摘 要： | 采用随机扩增多态性DNA(RAPD)分子标记技术对5个崖柏(Thuja sutchuenensis)天然居群的遗传变异进行研究。7个引物共检测44个位点,其中多态位点34个,占77.27%。与其它濒危植物相比,崖柏种内遗传多样性较为丰富。但居群间遗传分化占到33.68%。居群间遗传距离与空间距离不相关。根据崖柏居群遗传特征,初步探讨保护策略。
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| 关 键 词： | 崖柏 居群 遗传多样性 RAPD 保护 |
| 文章编号： | 1000-2286（2008）01-0068-05 |
| 收稿时间： | 2007-09-01 |
| 修稿时间： | 2007年9月1日 |
RAPD Analysis of the Genetic Diversity of a Critically Endangered Plant, Thuja sutchuenensis (Cupressaceae) |
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| LIU Jian-feng,XIAO Wen-fa.RAPD Analysis of the Genetic Diversity of a Critically Endangered Plant, Thuja sutchuenensis (Cupressaceae)[J].Acta Agriculturae Universitis Jiangxiensis,2008,30(1):68-72. |
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| Authors: | LIU Jian-feng XIAO Wen-fa |
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| Abstract: | RAPD molecular markers were used to assess genetic variation within and among five natural populations of Thuja sutchuenensis(Cupressaceae).Seven random primers detected 44 loci of which 34(77.27) were polymorphic.Compared with other endangered plants,the total genetic diversity of T.sutchuenensis was relative high.But the coefficient gene differentiation(Gst) among populations was up to 0.336 8.The genetic distances were also caculated,and did not correspond to geographic distance.The strategies of conservation for T.sutchuenensis based on genetic diversity analysis were proposed finally. |
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| Keywords: | Thuja sutchuenensis population genetic diversity RAPD conservation |
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