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Cd(Ⅱ)胁迫对盐藻生长、抗氧化系统及线粒体膜电位的影响
引用本文:向蓓,赵文,王媛,田丹. Cd(Ⅱ)胁迫对盐藻生长、抗氧化系统及线粒体膜电位的影响[J]. 大连水产学院学报, 2012, 27(1): 64-68
作者姓名:向蓓  赵文  王媛  田丹
作者单位:大连海洋大学辽宁省水生生物学重点实验室,辽宁大连,116023
基金项目:国家自然科学基金资助项目(40776065)
摘    要:研究了不同浓度的Cd(Ⅱ)(0、0.01、0.10、1.00 mmol/L)对盐藻Dunaliella salina生长、抗氧化系统及线粒体膜电位的影响。结果表明:在0.01 mmol/L和0.10 mmol/L的Cd(Ⅱ)胁迫下,盐藻的生长几乎不受影响,而1.00 mmol/L的Cd(Ⅱ)对盐藻生长有显著的抑制作用(P〈0.05);盐藻中丙二醛(MDA)的含量随着Cd(Ⅱ)处理浓度的变化先升高后降低;在1.00 mmol/L的Cd(Ⅱ)胁迫和诱导下,盐藻超氧化物歧化酶(SOD)的活性最大;总抗氧化力(T-AOC)仅在0.10 mmol/L的Cd(Ⅱ)胁迫下明显升高;各试验组盐藻线粒体膜电位受Cd(Ⅱ)胁迫的影响不明显。

关 键 词:Cd(Ⅱ)  盐藻  线粒体膜电位  超氧化物歧化酶  丙二醛  总抗氧化力

Effects of cadmium stress on the growth, mitochondrial membrane potential and antioxidative system in alga Dunaliella salina
XIANG Pei , ZHAO Wen , WANG Yuan , TIAN Dan. Effects of cadmium stress on the growth, mitochondrial membrane potential and antioxidative system in alga Dunaliella salina[J]. Journal of Dalian Fisheries University, 2012, 27(1): 64-68
Authors:XIANG Pei    ZHAO Wen    WANG Yuan    TIAN Dan
Affiliation:(Key Laboratory of Hydrobiology in Liaoning Province,Dalian Ocean University,Dalian 116023,China)
Abstract:Effects of cadmium(0,0.01,0.10,and 1.00 mmol/L) on the growth,antioxidant system and the mitochondrial membrane potential(ΔΨm) were studied in alga Dunaliella salina.There was no significant effect of cadmium on the growth of the alga at a concentration of 0.01 and 0.10 mmol/L Cd(Ⅱ),whereas the growth of the alga was significantly inhibited at Cd(Ⅱ) concentration of 1.00 mmol/L(Ⅱ).The content of malondialdehyde(MDA) in the cells was first found to be increased and then declined with increase in Cd(Ⅱ) level.The maximum activity of superoxide dismutase(SOD) was observed at Cd(Ⅱ) concentration of 1.00 mmol/L,and the alga had significantly higher total antioxidant capacity(T-AOC) only at Cd(Ⅱ) level of 0.10 mmol/L.However,there was no significant effect of Cd(Ⅱ) stress on the mitochondrial membrane potential(ΔΨm).
Keywords:Cd(Ⅱ )  Dunaliella salina  mitochondrial membrane potential  SOD  MDA  T-AOC
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