农杆菌介导不同基因型大豆品种子叶节遗传转化条件的研究

以黑农46、黑农53和黑农56的子叶节为转化受体,对农杆菌介导大豆子叶节遗传转化中的6-BA浓度、草丁膦浓度、侵染时间、共培养时间以及伸长培养基进行筛选,确定适合不同基因型的最佳转化条件。结果表明:黑农46、黑农53和黑农56的最佳6-BA诱导浓度分别为1.7、1.6和1.7 mg.L-1;最佳草丁膦筛选浓度分别为2.0、3.0和2.0 mg.L-1;同时确定了侵染时间、共培养时间和伸长培养基类型的最佳组合是侵染时间25 min、共培养时间3 d和Ⅲ型伸长培养基。

Optimization on Genetic Transformation Conditions of Agrobacterium-mediated Cotyledonary-nodes from Different Genotype Soybeans

Cotyledonary-nodes of Heinong 46,Heinong 53 and Heinong 56 were used as transformation receptors.Culture conditions including 6-BA concentration,glufosinate concentration,infection time,co-culture time and type of elongation medium were screened,in order to find the optimal conditions for Agrobacterium-mediated genetic transformation from different soybean genotypes.Result showed the optimal 6-BA induction concentrations for Heinong 46,Heinong 53 and Heinong 56 were 1.7,1.6 and 1.7 mg·L-1,respectively;the optimal concentrations of glufosinate for Heinong 46,Heinong 53 and Heinong 56 were 2.0,3.0,and 2.0 mg·L-1,respectively;the optimal combination of infection time,co-culture time and type of elongation medium was infection time 25 min,co-culture time 3 d and type Ⅲ of elongation medium.