野生大豆接种大豆疫霉根腐病菌后多酚氧化酶(PPO)活性变化

以8个对黑龙江省大豆疫霉菌1号优势生理小种抗感性不同的野生大豆为材料,对接种大豆疫霉菌游动孢子后野生大豆根、茎、叶中多酚氧化酶(Polyphenol Oxidase,PPO)活性变化情况进行了初步研究。结果表明:抗病野生大豆根和叶中PPO活性在整个病程均比相应对照增加,茎中PPO活性在病程的大部分阶段高于对照,且根中PPO活性高于茎和叶的PPO活性增幅;感病野生大豆根和叶中PPO活性在病程的大部分阶段低于对照。

Response of Polyphenol Oxidase Activity in Glycine soja Inoculated with Phytophthora sojae

Phytophthora root and stem rot of soybean(Phytophthora sojae)has become a serious damage to soybean production in China,especially in main soybean production region,Heilongjiang province.Polyphenol oxidase(PPO)is an important enzyme related to disease resistance in plants.However,there are few reports about response of PPO activity in wild soybeans infected with Phytophthora sojae.A total of eight wild soybeans including four resistant and four susceptible ones were inoculated with zoospores of P.sojae race 1 as treatment and those inoculated with water as control.The results showed that PPO activity in roots and leaves of resistant wild soybeans increased compared with those of control at the whole stage of pathogenetic process,and that in stems also increased at most stages of pathogenetic process.However,the PPO activity in roots and leaves of the four susceptible wild soybeans decreased at most stages of pathogenetic process.The results could lay theoretical basis on physiological and biochemical mechanism for disclosing wild soybean’s resistance to P.sojae.