|
|||||
|
|
| 6个地方猪品种(类群)GH基因的基因型分布研究 | |
| 引用本文： | 张依裕,胡尚,陈国宏,张海波.6个地方猪品种(类群)GH基因的基因型分布研究[J].河南农业科学,2008(12). |
| 作者姓名： | 张依裕 胡尚 陈国宏 张海波 |
| 作者单位： | 1. 贵州大学,动物科学学院,贵州,贵阳,550025 2. 贵阳市花溪区农业局,贵州,贵阳,550025 3. 扬州大学,动物科学与技术学院,江苏,扬州,225009 |
| 基金项目： | 贵州省自然科学基金 |
| 摘 要： | 采用PCR-RFLP技术对6个地方品种(类群)GH基因-119bp至+486bp区域进行扩增,分别用内切酶ApaⅠ和Hin6Ⅰ检测其多态性。结果表明:ApaⅠ酶切产生2个等位基因A和B,3种基因型AA,AB和BB,AA为优势基因型,A为优势等位基因;Hin6Ⅰ酶切产生3个等位基因C2,C3和C4,4种基因型C2C3,C2C4,C3C4和C4C4,C4C4为优势基因型,C4为优势等位基因。ApaⅠ和Hin6Ⅰ酶切基因型在猪群体间的分布不均匀,且差异都达到极显著水平(P<0.01)。 |
| 关 键 词： | 猪 生长激素基因 PCR-RFLP 限制性片断长度多态性 |
The Genotype Frequency of Porcine GH Gene in Six Local Breeds (Population) |
|
| ZHANG Yi-yu,HU Shang,CHEN Guo-hong,ZHANG Hai-bo.The Genotype Frequency of Porcine GH Gene in Six Local Breeds (Population)[J].Journal of Henan Agricultural Sciences,2008(12). | |
| Authors: | ZHANG Yi-yu HU Shang CHEN Guo-hong ZHANG Hai-bo |
| Abstract: | In this study,the polymorphisms from-119bp to +486bp of growth hormone gene in six local pig breeds(populations) were investigated by PCR-RFLP.2 alleles(A and B) and 3 genotypes(AA,AB and BB) were produced when the PCR products were digested by ApaⅠ,of which A and AA were predominant.When digested by Hin6Ⅰ,3 alleles(C2,C3 and C4) and 4 genotypes(C2C3,C2C4,C3C4 and C4C4) were produced,among which C4 and C4C4 were predominant allele or genotype.The results showed that the genotype distribution in six local breeds(populations) are significant different(P<0.01). |
| Keywords: | PCR-RFLP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |