丁内酯-I对绵羊卵母细胞体外成熟和受精的影响

绵羊卵母细胞经丁内酯-I(BL-I)抑制培养，然后转入成熟培养液培养不同时间，进行体外受精并观察胚胎发育情况。结果：解除8h抑制再培养8h组处于MⅡ期卵母细胞比率6.38％显著低于其它组别（P<0.01）。继续培养16h、20h的卵母细胞成熟率与对照组差异不显著，培养24h组卵母细胞成熟率(70.83%)稍高于对照组(66.18％)，但差异不显著(P>0.05)。培养16h、20h组囊胚率分别为2.99%、13.64％与对照组24.39%差异显著(P<0.05)，培养24h组的囊胚率20.33％与对照组差异不显著(P>0.05)。结论：BL-I对绵羊卵母细胞的成熟抑制作用是可逆的，经BL-I处理后未能提高绵羊卵母细胞体外受精和胚胎的整体发育能力。

Effect of Butyrolactone I on in-vitroMaturation and in-vitro Fertilization of Ovine Oocytes

In this study, the in-vitro maturation (IVM) and in-vitro fertilization (IVF) of ovine oocytes with inhibitor, BL-I, was investigated. Oocytes were cultured in vitro (IVC)among different durations after being inhibited for 8h. Results: MⅡ rate of the 8h IVC group was significantly lower than the other groups. There were no significant differences of the maturation rates among the 16h、20h IVC groups and the control one; the maturation rate (70.83%) of the 24h IVC group was higher than the control one (66.18％) ,but there were no significant differences between them (P>0.05). There were significant differences of the blastocyst rates among the 16h、20h IVC and control groups . Conclusion: The effect of inhibition by BL-I on IVM of ovine oocytes is reversible; and the inhibition treatment with BL-I on ovine IVM, can not improve the fertilization in-vitro and developmental competence of embryos.