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马铃薯试管块茎诱导过程中mRNA差异显示分析
引用本文:柳俊,田振东,宋波涛,谢从华.马铃薯试管块茎诱导过程中mRNA差异显示分析[J].中国马铃薯,2004,18(2):65-67.
作者姓名:柳俊  田振东  宋波涛  谢从华
作者单位:园艺植物生物学教育部重点实验室,国家蔬菜改良华中分中心,华中农业大学,湖北,武汉,445000
基金项目:国家自然科学基金资助项目(39970464).
摘    要:试验采用DD-PCR技术,对马铃薯试管块茎诱导初期的mRNA表达进行了差异显示分析。结果表明,块茎形成过程中,除了有新的基因表达开启外,还有一些基因表达关闭,同时还伴随着许多基因的表达强度增强,表明块茎形态建成是一系列基因协同作用的结果。

关 键 词:马铃薯  试管块茎  诱导  mRNA差异显示
文章编号:1672-3635(2004)02-065-03
修稿时间:2004年1月13日

mRNA DIFFERENTIAL DISPLAY DURING MICROTUBER INDUCTION OF POTATOES
LIU Jun,TIAN Zhen-dong,SONG Bo-tao,XIE Cong-hua Centre of Vegetable Improvement of Centre China,Huazhong Agricultural University,Wuhan ,China.mRNA DIFFERENTIAL DISPLAY DURING MICROTUBER INDUCTION OF POTATOES[J].Chinese Potato,2004,18(2):65-67.
Authors:LIU Jun  TIAN Zhen-dong  SONG Bo-tao  XIE Cong-hua Centre of Vegetable Improvement of Centre China  Huazhong Agricultural University  Wuhan  China
Institution:LIU Jun,TIAN Zhen-dong,SONG Bo-tao,XIE Cong-hua Centre of Vegetable Improvement of Centre China,Huazhong Agricultural University,Wuhan 430070,China)
Abstract:The expression mRNA of early stage of microtuber-inducing in different conditions was analyzed with mRNA differentiation display.The result indicated that some genes expressed while some others closed,with some gene increasing in expression during tuber initiation,suggesting that tuber formation was a result of activation of many genes.
Keywords:potato  microtuber  induction  mRNA differetial display
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