荆半夏叶柄外植体快繁技术研究

目的]通过组培的方法探索荆半夏形成生产用小块茎的最佳途径。方法]将叶柄躺接或竖接于不同的培养基诱导珠芽,然后将珠芽接入不同培养基并在不同光照条件下培养使其形成小块茎。结果]从珠芽形成之前的半夏小苗所取叶柄竖接至培养基MS+0.5mg/L BA+0.2 mg/L NAA中获得大量质量较好的珠芽,珠芽分开后接入培养基MS+0.05 mg/L BA+0.01 mg/L NAA中,采用10 h/d光照培养可获得适合于大田栽种的小块茎。结论]荆半夏叶柄经过适当的组培方法可在短时间内获得大量生产上使用的小块茎。

Study on the Rapid-Propagation Technical System with the Petioles of Pinellia Ternate Breit as Explants

The research aimed to find out the high-efficiency technical system for rapid propagation of Pinellia ternate Breit.The petioles cut from the different growth stages of Pinellia ternate Breit were cultured on different media.The buds forming from the petioles after a period of culture were separated and placed on different regeneration media.The result indicated that MS medium with 0.5 mg/L BA and 0.2 mg/L NAA was appropriate to stand the petioles before its shoot forming.More and better tubers can be induced through the shoot-culturing on MS medium with 0.05 mg/L BA 0.01 mg/L NAA under 10-hour illumination per day.The tubers,which can rapidly form from petioles by the method of tissue culture,can be well used as seeds in cultivation of pinellia ternate Breit.