香菇多糖的纯化和结构分析

香菇中提取到的水溶性粗多糖，用Ｓｅｖａｇ法结合酶法去蛋白，透析，甲醇分级得到２个多糖级分，分别命名为Ｌｅｎ１和Ｌｅｎ２。去蛋白后多糖经Ｓｅｐｈａｄｅｘ Ｇ－１００凝胶过滤层析纯化得精品多糖Ｌｅｎ３，经纸层析及凝胶过滤层析表现单一区带或单一峰。经酸水解后纸层析和气相色谱分析表明Ｌｅｎ２含甘露糖、葡萄糖，摩尔比为甘露糖：葡萄糖＝１０．５０：１．００，Ｌｅｎ３含木糖、甘露糖、葡萄糖，摩尔比为木糖：甘露糖：葡萄糖＝０．６７：２．４５：１．００。２００￣４００ｎｍ紫外扫描，无核酸和蛋白吸收峰。４０００￣６５０ｃｍ＾－１红外光谱扫描，呈多糖类特征吸收峰。

Purification and identification of polysaccharide from Lentinus edodes

Water soluble polysaccharide (lentinan) was crudely extracted from Lentinus edodes, then purified by the methanol fractionation with polysaccharide solution: methanol(v/v) 1: 0. 5 and 1: 1 respectively named ben 1 and ho 2. subsequently, the deproteinized polysaccharide was refined by gel filtration with Sephadex G-100 named Len 3. The finished products were identified by paper chromatography and gel filtration with Sephadex G-100. The compositions of ten 2 and Len 3 were separated by paper-and gas-chromatography. The results show that Len 2 is composed of mannose and glucose with a molar ratio of 10. 50: 1. 00, but Len 3 is composed of xylose, mannose and glucose with a molar ratio of 0.67:2 .45: 1 .00.