利用与抗稻瘟病基因Pi1连锁的MRG4766标记鉴定173份云南地方稻种

稻瘟病是世界上危害最严重的水稻病害之一,鉴定与发掘抗稻瘟病种质资源是抗稻瘟病育种的重要基础工作。本研究利用与抗稻瘟病基因Pi1连锁的SSR引物MRG4766对173份云南地方稻种抗性基因Pi1进行了检测,并选择其中30个材料进行了人工接种验证。结果表明,人工接种验证与分子鉴定结果完全相符,说明SSR引物MRG4766用于鉴定抗稻瘟病基因Pi1是可行的。分子标记检测表明,供试的173份材料中有64份含有抗稻瘟病基因Pi1,占36.99%;其中102份籼稻中有33份含有该基因,占32.35%,而71份粳稻中有31份含有该基因,占43.66%,粳稻含有抗稻瘟病基因Pi1的比例比籼稻要高。检测到含有Pi1基因的种质材料分布在云南省11个州市29个县(市),分布范围较为广泛。南部边缘水陆稻区分布频率为41.03%,滇南单双季籼稻区分布频率为32.69%,滇中一季粳稻籼稻区分布频率为35.29%、滇东北高原粳稻区为35.29%、滇西北高原粳稻区33.33%。

Rice Blast Resistance Gene Pi1 Identified by MRG4766 Marker in 173 Yunnan Rice Landraces

Rice Blast is one of the most serious rice diseases and caused great yield losses every year in the world.It had been proved that using resistant rice varieties would be the most effective way to control this disease,therefore,mining the resistant genes might be important foundational work in the breeding program.In the present study,we identified the existence of the Pi1 gene among the tested 173 landraces collected from Yunnan province by using the SSR marker MRG4766.The results showed that the Pi1 gene was found in 64 landraces,accounting for 36.99% in total of 173 landraces.33 of 102 indica landraces(32.35%) and 31 of 71 japonica landraces(43.66%) both contained Pi1 gene.The landraces harboring the Pi1 gene were distributed in 29 counties of 11 prefectures in Yunnan province.The distribution frequency of Pi1 gene was higher in paddy-upland rice region in South margin of Yunnan(41.03%) than that of in single and or double indica cropping region in south of Yunnan(32.69%).The distribution frequency of Pi1 gene was 35.29% in single indica and or japonica cropping region in center of Yunnan,35.29% in japonica cropping region in northeast of Yunnan and 33.33% in japonica cropping region in northwest highland of Yunnan.