| 蛹虫草超氧化物歧化酶的分离纯化及性质研究 |
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| 引用本文： | 苏瑛,唐咏,王成春. 蛹虫草超氧化物歧化酶的分离纯化及性质研究[J]. 安徽农业科学, 2005, 33(8): 1388-1389 |
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| 作者姓名： | 苏瑛 唐咏 王成春 |
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| 作者单位： | 沈阳农业大学基础部,辽宁,沈阳,110161;沈阳农业大学生物技术学院,辽宁,沈阳,110161 |
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| 摘 要： | 以蛹虫草为材料,经过硫酸铵盐析、SephedexG!75柱层析和DEAE!52柱层析,得到纯化的超氧化物歧化酶(SOD),经聚丙烯酰胺凝胶电泳显示单一蛋白区带,此酶比活力为17855.73U/mg,纯化倍数为53.7,回收率为21.8%。该酶在40℃保温150min,活性不变;在pH值5.0～9.0有较好的稳定性。该酶每亚基含1原子铁,对H2O2和乙醇-氯仿溶液敏感。
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| 关 键 词： | 蛹虫草SOD 分离纯化 性质 |
| 文章编号： | 0517-6611（2005）08-1388-02 |
| 收稿时间： | 2005-05-16 |
| 修稿时间： | 2005-05-16 |
Purification and Characterization of Superoxide Dismutase from Chinese Caterpillar Fungus |
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| SU Ying et al. Purification and Characterization of Superoxide Dismutase from Chinese Caterpillar Fungus[J]. Journal of Anhui Agricultural Sciences, 2005, 33(8): 1388-1389 |
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| Authors: | SU Ying et al |
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| Abstract: | Superoxide dismutase (SOD) from Chinese caterpillar fungus was separated and purified through ammonium sulfate precipitation andcolumn chromatographywith SephadexG-75 gel filtration and DEAE-52 ion exchange. The purified enzyme showed a single band on PAGE. Thespecific activity was 17 855.73 U/mg, the SOD enzyme was 57.3-fold purified, 21.8 % of the SOD activity was recovered. The SOD from Chinesecaterpillar fungus had good heat (40 ℃, 150 min) and acid- base stability in pH 5.0￣9.0, in which the activity of SOD did not decline. Everysubunit ofthis SODcontained one iron atom. The enzyme was sensitive toH2O2and Tsuchihashi. |
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| Keywords: | Chinese caterpillar fungus Superoxide dismutase Purification Character |
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