| 牛生长激素BGH基因的克隆及其真核表达载体pEGFP-N1-BGH的构建 |
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| 引用本文： | 韦光辉,徐廷生,雷雪芹,索剑飞. 牛生长激素BGH基因的克隆及其真核表达载体pEGFP-N1-BGH的构建[J]. 河南农业科学, 2010, 0(1) |
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| 作者姓名： | 韦光辉 徐廷生 雷雪芹 索剑飞 |
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| 作者单位： | 河南科技大学,动物科技学院,河南,洛阳,471003 |
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| 基金项目： | 河南省基础与前沿技术研究计划项目 |
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| 摘 要： | 采用逆转录聚合酶链式反应(RT-PCR)技术,从11月龄的牛脑垂体组织中扩增出BGH基因片段,通过DNA重组技术将该基因片段重组于pEGFP-N1真核表达载体上,构建pEGFP-N1-BGH重组质粒。通过PCR扩增、酶切电泳分析和测序的方法对重组DNA进行鉴定,结果表明,成功构建了pEGFP-N1-BGH真核表达载体。
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| 关 键 词： | 牛生长激素基因 克隆 真核表达载体pEGFP-N1 载体构建 |
Cloning of BGH Gene and Construction of a Eukaryotic Expression Vector pEGFP-N1-BGH |
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| WEI Guang-hui,XU Ting-sheng,LEI Xue-qin,SUO Jian-fei. Cloning of BGH Gene and Construction of a Eukaryotic Expression Vector pEGFP-N1-BGH[J]. Journal of Henan Agricultural Sciences, 2010, 0(1) |
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| Authors: | WEI Guang-hui XU Ting-sheng LEI Xue-qin SUO Jian-fei |
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| Abstract: | The bovine growth hormone(BGH) gene was amplified from the tissue of calf brain pituitary by RT-PCR and cloned into the eukaryotic expression vector pEGFP-N1.The recombinants were further identified by PCR amplification,enzyme digestion and DNA sequencing.The results showed that the eukaryotic expression vector pEGFP-N1 was successfully constructed. |
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| Keywords: | BGH gene Cloning Eukaryotic expression vector pEGFP-N1 Vector construction |
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