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| 云南省核桃品种遗传多样性的FISH-AFLP分析 | |
| 引用本文： | 宁德鲁,马庆国,张雨,王滑,陆斌,裴东. 云南省核桃品种遗传多样性的FISH-AFLP分析[J]. 林业科学研究, 2011, 24(2): 189-193 |
| 作者姓名： | 宁德鲁 马庆国 张雨 王滑 陆斌 裴东 |
| 作者单位： | 1. 云南省林业科学院经济林研究所,云南,昆明,650204 2. 中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京,100091 3. 云南省林业技术推广总站,云南,昆明,650224 |
| 基金项目： | 林业公益性行业科研专项经费项目:1.核桃优异种质资源保存与开发利用技术研究(200704046);2.核桃集约及可持续生产关键技术研究(201004048) |
| 摘 要： | 采用FISH-AFLP技术,选取8对E+3和M+3引物组合,对云南省林木品种审定委员会审(认)定的17个核桃品种(无性系)进行了基因组DNA分子水平上的检测,实验结果共获得987条可统计的条带,其中942条呈多态性,多态性带百分率达95.44%;17份供试核桃样品的遗传相似系数范围为0.606 9～0.796 4,平均... |
| 关 键 词： | 核桃 品种 遗传多样性 FISH-AFLP 聚类分析 |
| 收稿时间： | 2010-07-05 |
FISH-AFLP Analysis of Genetic Diversity on Walnut Cultivars in Yunnan Province |
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| NING De-lu,MA Qing-guo,ZHANG Yu,WANG Hu,LU Bin and PEI Dong. FISH-AFLP Analysis of Genetic Diversity on Walnut Cultivars in Yunnan Province[J]. Forest Research, 2011, 24(2): 189-193 | |
| Authors: | NING De-lu MA Qing-guo ZHANG Yu WANG Hu LU Bin PEI Dong |
| Affiliation: | Research Institute of Economic Forestry, Yunnan Academy of Forestry, Kunming 650204, Yunnan, China;;Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;;Research Institute of Economic Forestry, Yunnan Academy of Forestry, Kunming 650204, Yunnan, China;;Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;;Yunnan General Station of Forestry Technology Extension Station, Kunming 650224, Yunnan, China;Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China; |
| Abstract: | The genetic diversities of 17 walnut cultivars(Juglans sigillata Dode) authorized or approved by Yunnan Province Forest Tree Cultivar Registration Committee were investigated with FISH-AFLP technical system. Eight pairs of E+3/M+3 primers were used to amplify the genomic DNA. The results showed that 987 AFLP bands were obtained, 942 of them were polymorphic markers and the average percent of polymorphic bands was 95.44%. The genetic similarity indexes among all the 17 cultivars are ranged from 0.606 9 to 0.796 4 with an average of 0.734 9. These investigations would be significant in utilizing walnut germplasm resources and cultivating new varieties. |
| Keywords: | walnut(Juglans sigillata Dode) cultivar genetic diversity FISH-AFLP cluster analysis |
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