苹果离体新梢外植体继代培养次数对其再生的影响

 对不同继代培养次数的富士、金冠、乔纳金苹果(Malus domestica Borkh. ) 组培离体新梢外植体分别进行新梢增殖倍数、生根和叶片不定芽再生能力研究。结果表明, 3个品种无菌繁殖系建立后第5次继代培养的离体新梢外植体的增殖倍数、生根和叶片不定芽再生能力均较低; 随继代次数增加, 以上各种器官再生能力稳定在较高水平, 继代76、164代的富士及76、84、116代的金冠和乔纳金新梢外植体间均无明显差别。说明苹果新梢外植体达到离体培养状态后经20年100余次继代培养, 其器官再生能力没有显著变化。

Effects of Subculture Times on Organogenesis Characteristics of Apple in Vitro Shoot Explants

The organogenesis characteristics of apple(Malus domestica Borkh.) shoot explants repeatedly subcultured in vitro were examined by analysis of the shoot multiplication,rooting,and adventitious bud regeneration rate from leaves.The results showed low propagation ability for the shoot explants subcultured in vitro for five times or less at the beginning of inoculation in terms of its shoot multiplication,rooting,and adventitious bud regeneration rate from leaves.With the increasing number of subculturing times,the regeneration ability of the organs above enhanced and remaind at a high level.There were no significant differences among the shoot plantlets being subcultured for 76 and 164 times in Fuji,and for 76,84,and 116 times in Golden Delicious and Jonagold in the aspects of above.It is concluded that the apple shoot plantlets repeatedly subcultured in vitro for more than one hundred times for twenty years retain the organogenesis characteristics as usual and can still be used for the plant micropropagation and gene transformation.