Comparative genomic analysis of eight Leptospira strains from Japan and the Philippines revealing the existence of four putative novel genomic islands/islets in L. interrogans serovar Lai strain 56601 |
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| Institution: | 1. Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, Kita-20, Nishi-10, Kita-ku, Sapporo 001-0020, Hokkaido, Japan;2. National Research Center for Protozoan Diseases, Obihiro University, Nishi-2-13, Inada-cho, Obihiro 080-8555, Hokkaido, Japan;3. Department of Bacteriology, National Institute of Infectious Disease, 1-23-1 Toyama, Shinju-ku 162-8640, Tokyo, Japan;1. Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran;2. Department of Ophthalmology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran;3. Department of Parasitology and Mycology, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran;4. Biostatistics Department, Mazandaran University of Medical Sciences, Sari, Iran;1. Centro de Biotecnología-FEMSA, Tecnologico de Monterrey, Campus Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey, NL 64849, Mexico;2. Department of Chemical and Biomolecular Engineering, University of Houston, 4800 Calhoun Rd., Houston, TX 77004, United States;1. State University of Londrina, Rod. Celso Garcia Cid, km 380, 86057-970 Londrina, PR, Brazil;2. University of the Ryukyus, Okinawa, Japan;3. Murata Animal Hospital, Mobara, Chiba, Japan;1. Division of Infectious Disease, Department of Internal Medicine, Tungs’ Taichung MetroHarbor Hospital, Taichung, Taiwan;2. Graduate Institute of Microbiology and Public Health, National Chung Hsing University, Taichung, Taiwan;3. Infection Control Office, Tungs’ Taichung MetroHarbor Hospital, Taichung, Taiwan;4. Department of Medical Research, Tungs’ Taichung MetroHarbor Hospital, Taichung, Taiwan |
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| Abstract: | Leptospirosis is one of the most widespread zoonotic diseases worldwide and can be considered an emerging health problem to both human and animal. Despite the importance of the disease, complete genome sequences are currently available for only three Leptospira interrogans strains: 56601, Fiocruz L1-130, and IPAV. Therefore, intra- and inter-species comparative genomic analyses of Leptospira are limited. Here, to advance current knowledge of the genomic differences within Leptospira species, next-generation sequencing technology was used to examine the genomes of eight L. interrogans strains belonging to six different serogroups isolated from humans and dogs in Japan and the Philippines. The genomic sequences were mapped to that of the reference strain, L. interrogans serovar Lai strain 56601. The results revealed the presence of four novel genomic islands/islets (GIs) in strain 56601. This study provides a deeper insight into the molecular basis and evolutionary perspective of the virulence of leptospires. |
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| Keywords: | Genomic island Leptospirosis Next-generation sequencer |
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