Determination of phospholipids in olive oil by 31P NMR spectroscopy |
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Authors: | Hatzakis Emmanuel Koidis Anastasios Boskou Dimitrios Dais Photis |
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Affiliation: | NMR Laboratory, Department of Chemistry, University of Crete, Voutes Campus, P.O. Box 2208, 71003 Heraklion, Crete, Greece. |
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Abstract: | A nondestructive analytical method based on NMR spectroscopy was developed for the determination of phospholipids in olive oil. The phospholipids extracted from virgin olive oil with a mixture of ethanol/water (2:1 v/v) were identified and quantified by high resolution (31)P NMR spectroscopy. The main phospholipids found in olive oil were phosphatidic acid, lyso-phosphatidic acid, and phosphatidylinositol. Validation of the (31)P NMR methodology for quantitative analysis of phospholipids in olive oil was performed. Sensitivity was satisfactory with detection limits of 0.25-1.24 mumol /mL. In addition, the composition of fatty acids in phospholipids model compounds and those in olive oil samples was estimated by employing one- and two-dimensional (1)H NMR. The results indicated that the fatty acid composition in phospholipids and triacylglycerols of olive oil was similar. |
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