Parathyroid hormone-related protein (PTHrP) produced by dog lymphoma cells |
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| Authors: | Kubota Akiko Kano Rui Mizuno Takuya Hisasue Masaharu Moore Peter F Watari Toshihiro Tsujimoto Hajime Hasegawa Atsuhiko |
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| Institution: | Department of Pathobiology, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan. |
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| Abstract: | Parathyroid hormone-related protein (PTHrP) was investigated in a canine lymphoma case with hypercalcemia by means of immunoradiomentric assay (IRMA) and molecular analysis. The plasma calcium level of the patient dog was 13.7 mg/dl. The PTHrP concentration examined by IRMA was 6.1 pmol/L in the plasma sample from the dog, but it was undetectable (< 1.1 pmol/L) in plasma samples from 4 lymphoma cases without hypercalcemia or 5 normal dogs. The PTHrP concentration examined in the culture supernatant of the lymphoma cells from this case was 1.3 pmol/L, whereas those of the lymphoma cells from a lymphoma case without hypercalcemia was undetectable. PTHrP mRNA was clearly detected not only in the lymphoma cells from this dog with hypercalcemia but also in lymphoma cells from 4 lymphoma cases without hypercalcemia and 2 canine lymphoma cell lines. |
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