| 无籽刺梨多倍体诱导试验初报 |
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| 引用本文： | 李斌, 林源, 辛亚龙, 唐军荣, 尹丽莎, 韩国伟, 辛培尧. 无籽刺梨多倍体诱导试验初报[J]. 西南林业大学学报, 2016, 36(5): 27-31.doi:10.11929/j.issn.2095-1914.2016.05.005 |
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| 作者姓名： | 李斌 林源 辛亚龙 唐军荣 尹丽莎 韩国伟 辛培尧 |
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| 作者单位： | 1. 西南林业大学云南省高校林木遗传改良与繁育重点实验室，云南 昆明 650224；;2. 西南林业大学国家林业局西南地区生物多样性保育重点实验室，云南 昆明 650224；;3. 大理农林职业技术学院，云南 大理 671003 |
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| 基金项目： | 云南省林学一级学科博士点建设项目；西南林业大学云南省高校林木遗传改良与繁育重点实验室开放基金项目；云南省省院省校教育合作咨询共建重点学科项目 (211015) 资助。
摘要:采用育种技术对无籽刺梨进行多倍体诱导，以期获得无籽刺梨多倍体植株。以秋水仙素作为诱导剂，二倍体组培苗为材料，比较不同的预培养时间、处理时间及秋水仙素浓度对无籽刺梨染色体加倍的诱导效果。结果表明：无籽刺梨茎段在分化培养基上预培养1d后，继而用含300mg/L秋水仙素溶液浸泡处理12h，再进行分化培养的诱导效果最佳，其诱导变异率达256%；无籽刺梨多倍性植株同质化培养的最佳次数为6次。对变异植株根尖细胞进行染色体计数后发现，部分变异植株的根尖细胞染色体为2n=4x=28，为四倍体。部分植株同时存在2n=2x=14和2n=4x=28两种倍性细胞，为嵌合体。 |
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| 摘 要： | 采用育种技术对无籽刺梨进行多倍体诱导，以期获得无籽刺梨多倍体植株。以秋水仙素作为诱导剂，二倍体组培苗为材料，比较不同的预培养时间、处理时间及秋水仙素浓度对无籽刺梨染色体加倍的诱导效果。结果表明：无籽刺梨茎段在分化培养基上预培养1d后，继而用含300mg/L秋水仙素溶液浸泡处理12h，再进行分化培养的诱导效果最佳，其诱导变异率达256%；无籽刺梨多倍性植株同质化培养的最佳次数为6次。对变异植株根尖细胞进行染色体计数后发现，部分变异植株的根尖细胞染色体为2n=4x=28，为四倍体。部分植株同时存在2n=2x=14和2n=4x=28两种倍性细胞，为嵌合体。
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| 关 键 词： | 无籽刺梨 多倍体诱导 浸泡处理 染色体数 组织培养 |
| 收稿时间： | 2016-04-15 |
Preliminary Report on PolyPloidy Induction of Rosa sterilis |
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| Li Bin1, 2, Lin Yuan3, Xin Yalong1, Tang Junrong1, 2 and Yin Lisha1. Preliminary Report on PolyPloidy Induction of Rosa sterilis[J]. Journal of Southwest Forestry University, 2016, 36(5): 27-31.doi:10.11929/j.issn.2095-1914.2016.05.005 |
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| Authors: | Li Bin1 2 Lin Yuan3 Xin Yalong1 Tang Junrong1 2 Yin Lisha1 |
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| Affiliation: | 1. Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming Yunnan 650224,China; ;2. Key Laboratory of Biodiversity Conservationin Southwest Region of State Forestry Administration, Kunming Yunnan 650224,China; ;3. Dali Vocational and Technical College of Agriculture and Forestry, Dali Yunnan 671003,China |
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| Abstract: | The polyploidy breeding technology was used to induce Rosa sterilis polyploidy, for the obtaining of Rsterilis polyploidy plants. The colchicine was used as induction dose, diploid cuvette seedling of Rsterilis as material, to compare induction effect on chromosome doubling in different preculture time, different colchicine concentration and different treatment time. The results showed that after preculture in differentiation medium for 1d, and soaking in 300mg/L colchicine solution for 12h, then differentiation culture in differentiation medium, the induction effect was better, and the mutation rate was 256%. The best culture frequency of homogenization was 6. After observing the chromosome number in root tip of mutation plants, it was found that chromosome number in some tetraploid plants cells was 2n=4x=28,while cells with both 2n=2x=14 and 2n=4x=28 in some chimera plants. |
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| Keywords: | Rosa sterilis polyploid induction soaking treatment choromosome number tissue culture |
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