| 云南特有火把梨UFGT基因片段的克隆与序列分析 |
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| 引用本文： | 孟富宣, 周军, 王大玮, 陶磅, 董娇, 徐世宏, 段淋渊. 云南特有火把梨UFGT基因片段的克隆与序列分析[J]. 西南林业大学学报, 2016, 36(1): 21-27.doi:10.11929/j.issn.2095-1914.2016.01.004 |
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| 作者姓名： | 孟富宣 周军 王大玮 陶磅 董娇 徐世宏 段淋渊 |
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| 作者单位： | 1.云南省高校林木遗传改良与繁育重点实验室，云南 昆明 650224；;2.云南省农业科学院热区生态农业研究所，云南 元谋 651300；;3.西南山地森林资源保育与利用省部共建教育部重点实验室，云南 昆明 650224；;4.西南林业大学林学院，云南 昆明 650224；;5.云南省农业科学院园艺作物研究所,云南 昆明 650000；;6. 中华全国供销合作总社昆明食用菌研究所，云南 昆明 650223 |
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| 基金项目： | 云南省教育厅科学研究基金项目 (2012J050)资助；西南林业大学科研启动基金资助。
摘要:为研究UFGT基因对红皮梨花色素苷合成的调控机制，以云南特有火把梨为试材，克隆得到UFGT的基因片段，运用生物信息学分析软件对该基因序列进行分析。结果表明：UFGT基因片段长1440bp，编码479个氨基酸的蛋白质；该氨基酸序列与沙梨、西洋梨、白梨、苹果和樱桃李的同源性分别为99%、96%、94%、91%和72%；蛋白无跨膜结构，不存在导肽和信号肽，属于非分泌蛋白，定位在胞液；结构功能域分析显示该片段含典型的GT1_Gtf_Like功能域和1个GTB型超家族蛋白的典型结构。 |
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| 摘 要： | 为研究UFGT基因对红皮梨花色素苷合成的调控机制，以云南特有火把梨为试材，克隆得到UFGT的基因片段，运用生物信息学分析软件对该基因序列进行分析。结果表明：UFGT基因片段长1440bp，编码479个氨基酸的蛋白质；该氨基酸序列与沙梨、西洋梨、白梨、苹果和樱桃李的同源性分别为99%、96%、94%、91%和72%；蛋白无跨膜结构，不存在导肽和信号肽，属于非分泌蛋白，定位在胞液；结构功能域分析显示该片段含典型的GT1_Gtf_Like功能域和1个GTB型超家族蛋白的典型结构。
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| 关 键 词： | 火把梨 UFGT 基因克隆 生物信息学 |
| 收稿时间： | 2015-05-12 |
Cloning and Analysis of UFGT Gene Sequence from Pyrus pyrifolia cv. Huobali of Specific in Yunnan |
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| Meng Fuxuan1, 2, 3, 4, Zhou Jun1, 3 and 4. Cloning and Analysis of UFGT Gene Sequence from Pyrus pyrifolia cv. Huobali of Specific in Yunnan[J]. Journal of Southwest Forestry University, 2016, 36(1): 21-27.doi:10.11929/j.issn.2095-1914.2016.01.004 |
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| Authors: | Meng Fuxuan1 2 3 4 Zhou Jun1 3 4 |
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| Affiliation: | 1. Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province, Kunming Yunnan 650224, China；;2. Institute of Tropical Eco agricultural Sciences, Yunnan Academy of Agricultural, Yuanmou Yunnan 651300, China;;3. Key Laboratory for Forest Resource Conservation and Use in the Southwest Mountains of China, Kunming Yunnan 650224, China；;4. College of Forestry, Southwest Forestry University, Kunming Yunnan 650224, China；;5.Horticultural Research Institute Yunnan Acudemy of Agricultural Sciences,Kunming Yunnan,650000,China;;6. Kunming Edible Fungi Institute, All China Federation of Supply and Marketing Cooperatives, Kunming Yunnan 650223, China |
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| Abstract: | In order to research UFGT gene for regulation mechanism of anthocyan synthesis in red pear, Pyrus pyrifolia cv. Huobali of specific in Yunnan was used as experimental material and the cDNA fragment of UFGT gene was obtained by homologous cloning. Then, bioinformatics analysis of UFGT gene and its deduced protein was done by using the related biological software. The results showed that length of UFGT is 1 440 bp, and it encodes 479 amino acids. The homology at amino acids level of UFGT with UFGT in Pyrus pyrifolia (AFQ92055)、Pyrus communis (AGL81353)、Pyrus bretschneideri (AGZ15304)、Malus domestica (XP_008357063) and Prunus cerasifera (AKV89253) are 99%, 96%, 94%,91% and 72%, respectively. The results of amino acid sequence analysis indicated that the UFGT had no signal peptide and trans membrane domain, belonged to the non secreted protein and located in cytosol. The protein predication of UFGT showed that there were a function domains GT1_Gtf_Like and typical structure of GTB super family. |
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| Keywords: | Pyrus pyrifolia cv. Huobali UFGT gene cloning bioinformatics |
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