| 疑似多倍体小木漆的倍性鉴定 |
| |
| 引用本文： | 韩国伟,李旦,赵月明,田斌,和润喜,欧光龙,尹五元,何承忠.疑似多倍体小木漆的倍性鉴定[J].西南林业大学学报,2016,36(3):7-11. |
| |
| 作者姓名： | 韩国伟 李旦 赵月明 田斌 和润喜 欧光龙 尹五元 何承忠 |
| |
| 作者单位： | 1. 西南林业大学云南省高校林木遗传改良与繁育重点实验室，云南 昆明 650224; ;2. 西南林业大学西南地区生物多样性保育国家林业局重点实验室，云南 昆明 650224;; 3. 西南林业大学云南生物多样性研究院，云南 昆明 650224; ;4. 西南林业大学西南山地森林资源保育与利用省部共建教育部重点实验室，云南 昆明 650224 |
| |
| 基金项目： | 云南省教育厅重大专项（ZD2013014）资助；西南林业大学云南省省级重点学科 （林学） 建设项目资助；西南林业大学大学生创新基金项目 （C1475） 资助；西南林业大学科研启动基金项目 （111409） 资助；西南地区生物多样性保育国家林业局重点实验室开放基金资助。
摘要:以云南省昭通市疑似多倍体小木漆为试材，采用二倍体漆树为对照，通过叶片下表皮气孔观察、流式细胞仪倍性分析、基因组大小测定3种方法对其倍性进行鉴定。结果表明：疑似多倍体小木漆气孔密度为二倍体漆树的052倍，气孔长度为二倍体漆树的138倍，保卫细胞内叶绿体的个数为二倍体漆树的173倍。经流式细胞仪检测分析，疑似多倍体小木漆体细胞倍性为二倍体漆树的15倍，其基因组大小 (2C DNA含量) 平均为 （122 ± 0. 06）pg，而二倍体漆树的基因组大小 (2C DNA含量) 平均为 （096 ± 004）pg，基因组大小之比为127∶1。综上所述，采集的疑似多倍体小木漆为天然三倍体漆树。 |
| |
| 摘 要： | 以云南省昭通市疑似多倍体小木漆为试材，采用二倍体漆树为对照，通过叶片下表皮气孔观察、流式细胞仪倍性分析、基因组大小测定3种方法对其倍性进行鉴定。结果表明：疑似多倍体小木漆气孔密度为二倍体漆树的052倍，气孔长度为二倍体漆树的138倍，保卫细胞内叶绿体的个数为二倍体漆树的173倍。经流式细胞仪检测分析，疑似多倍体小木漆体细胞倍性为二倍体漆树的15倍，其基因组大小 (2C DNA含量) 平均为 （122 ± 0. 06）pg，而二倍体漆树的基因组大小 (2C DNA含量) 平均为 （096 ± 004）pg，基因组大小之比为127∶1。综上所述，采集的疑似多倍体小木漆为天然三倍体漆树。
|
| 关 键 词： | 小木漆 倍性 基因组大小 流式细胞仪 |
| 收稿时间： | 2015-12-26 |
Ploidy Identification of Pseudo Polyploidy in Toxicodendron vernicifluum |
| |
| Han Guowei,, Li Dan, Zhao Yueming,, Tian Bin, He Runxi,.Ploidy Identification of Pseudo Polyploidy in Toxicodendron vernicifluum[J].Journal of Southwest Forestry University,2016,36(3):7-11. |
| |
| Authors: | Han Guowei Li Dan Zhao Yueming Tian Bin He Runxi |
| |
| Institution: | 1. Key Laboratory for Forest Geneticand Tree Improvement & Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming Yunnan 650224, China; ;2. Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Southwest Forestry University, Kunming Yunnan 650224, China;; 3. Yunnan Academy of Biodiversity, Southwest Forestry University, Kunming Yunnan 650224, China; ;4. Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming Yunnan 650224, China |
| |
| Abstract: | With a pseudo polyploidy of Toxicodendron vernicifluum in Zhaotong, Yunnan province as the experimental materials, and the diploid Tvernicifluum as the control group, ploidy were determined by observation of the stomotal characteristic of leaf lower epidermis, analysis of flow cytometry and measurement of genome size. The results showed that the stomata density of pseudo polyploidy of Tvernicifluum was 052 times, the length of stomata was 138 times, and the number of chloroplast in the guard cells was 173 times compared with diploid Tvernicifluum, respectively. According to the results by flow cytometry analysis, the ploidy of somatic cell in pseudo polyploidy of Tvernicifluum was 15 times to that of the diploid Tvernicifluum, the average genome sizes (the content of 2C DNA) of pseudo polyploidy of Tvernicifluum and diploid Tvernicifluum was about (122 ± 006)pg and (096 ± 004)pg, respectively, and the average genome sizes of pseudo polyploidy of Tvernicifluum was 127 times to diploid Tvernicifluum. Above all, it could be concluded that the pseudo polyploidy of Tvernicifluum was a natural triploid lacquer. |
| |
| Keywords: | Toxicodendron vernicifluum ploidy genome size flow cytometry |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《西南林业大学学报》浏览原始摘要信息 |
| 点击此处可从《西南林业大学学报》下载免费的PDF全文 |
|
