全基因组预测枯草芽孢杆菌BEST195的分泌蛋白

枯草芽孢杆菌BEST195作为纳豆菌的一种，是纳豆生产的重要菌株之一。利用SignalP、ProtComp、TMHMM、Phobius、LipoP、TatP等预测程序对该菌中4456条蛋白质序列进行分泌蛋白找寻，同时，对上述分泌蛋白的氨基酸分布、信号肽长度大小、信号肽切割位点等性质进行分析。结果表明：枯草芽孢杆菌含有分泌蛋白102个，其氨基酸长度、信号肽长度与植物病原菌不同；信号肽切割位点属于A X A类型，与其他已经报道的植物病原真菌、细菌以及卵菌中分泌蛋白信号肽切割位点一致。通过上述生物信息学分析方法有效地实现了枯草芽孢杆菌BEST195分泌蛋白的预测，分泌蛋白的信号肽切割位点具有物种保守型特点。

Prediction for Secreted Proteins from Bacillus subtilis subsp. natto BEST195 Genome

Bacillus subtilis subsp. natto BEST195 is one of the important strains produced natto. To identify the secreted protein from Bsubtilis and clearly it′s characteristic. 4456 protein sequences in Bsubtilis was analyzed to find the secreted protein using the program including SignalP, ProtComp, TMHMM, Phobius, LipoP and TatP. Meanwhile, the distribution of amino acids, the length of signal peptide as well as the signal peptide cleavage site of secreted protein was analyzed. 102 secrete proteins were found in Bsubtilis. And the length of amino acids and the signal peptide were different the plant pathogens. The signal peptide cleavage site belongs to AXA type, which was same as other plant pathogenic fungi, bacteria and oomycete. Through the above bioinformatics analysis, the predicted secreted proteins can effectively achieve in Bsubtilis, and the type of signal peptide cleavage site have consistent with other secreted proteins from different species.