| 水稻细菌性条斑病菌和白叶枯病菌的多重PCR检测体系开发 |
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| 引用本文： | 岳凯, 高雪, 曹红云, 等. 水稻细菌性条斑病菌和白叶枯病菌的多重PCR检测体系开发[J]. 云南农业大学学报（自然科学）, 2016, 31(5): 773-778. DOI: 10.16211/j.issn.1004-390X(n).2016.05.001 |
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| 作者姓名： | 岳凯 高雪 曹红云 谷安宇 徐雨然 徐烨 刘雅婷 李小林 |
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| 作者单位： | 1.云南农业大学 农学与生物技术学院, 云南 昆明 650201;2.云南文山市新街乡农业技术推广站, 云南 文山 663008;3.云南省农业科学院 粮食作物研究所, 云南 昆明 650205;4.云南农业大学 植物保护学院, 云南 昆明 650201 |
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| 摘 要： | 本研究设计水稻细菌性条斑病菌和水稻白叶枯病菌的专化性引物，建立了可同时检测这两种细菌的多重PCR检测体系。通过体系优化、特异性和灵敏度检查，结果表明：两对专化性引物X-b和X-t具有较高的特异性和灵敏度，对水稻细菌性条斑病菌液和水稻白叶枯病菌液的检测灵敏度分别为104 cfu/mL和105 cfu/mL；利用本研究建立的多重PCR体系，成功地从50种市售水稻种子中检测出1个水稻品种带有水稻细菌性条斑病菌，1个水稻品种水稻白叶枯病菌。本研究建立了多重PCR检测体系，为检疫部门控制这两种检疫性种传病害的入侵提供了有效检测技术和手段。
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| 关 键 词： | 水稻细菌性条斑病菌 水稻白叶枯病菌 多重PCR体系 |
| 收稿时间： | 2015-07-02 |
| 修稿时间： | 2016-05-04 |
Development of Multiplex PCR Detection System for Xanthomonas oryzae pv. oryzicola and Xanthomonas oryzae pv. oryzae |
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| YUE Kai, GAO Xue, CAO Hongyun, et al. Development of Multiplex PCR Detection System for Xanthomonas oryzae pv. oryzicola and Xanthomonas oryzae pv. oryzae[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2016, 31(5): 773-778. DOI: 10.16211/j.issn.1004-390X(n).2016.05.001 |
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| Authors: | YUE Kai GAO Xue CAO Hongyun GU Anyu XU Yuran XU Ye LIU Yating LI Xiaolin |
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| Affiliation: | 1.College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China;2.The Xinjie Town of Wenshan Agriculture Technology Extension Station, Wenshan 663008, China;3.Institute of Food Crops, Yunnan Academy of Agricultural Sciences Research, Kunming 650205, China;4.College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China |
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| Abstract: | In this study, a multiplex PCR detection system was established for Xanthomonas oryzae pv. oryzicola and Xanthomonas oryzae pv. oryzae by designing two specific primers. Through system optimization, specificity and sensitivity tests, the results showed that two pairs of specific primers had a high specificity and sensitivity, and the detection sensitivity of X.oryzae pv. oryzicola and X.oryzae pv. oryzae were 104 cfu/mL and 105 cfu/mL,respectively. Using the multiple PCR system established in this study, a rice variety seed with X. oryzae pv. oryzicola and another rice variety seed with X. oryzae pv. oryzae were successfully detected from 50 kinds of commercially available rice seeds. A multiplex PCR detection system established in this study provides the effective detection technology and means for the quarantine department to control the invasion of these two kinds of quarantine seedborne diseases. |
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| Keywords: | Xanthomonas oryzae pv. oryzicola Xanthomonas oryzae pv. oryzae multiple PCR systems |
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