杂种白杨717 1B4组培技术研究

为保持杂种白杨717 1B4的优良性状，解决生根困难瓶颈，建立了生理年龄一致性和容器化生根炼苗的组织培养繁育技术。结果表明：外植体为母株根蘖的根际萌芽，以有效氯10%的乙氰脲酸钠溶液灭菌25min，接种在MS培养基上效果最好，再生不定芽达7591%；以再生不定芽3个以上的外植体进行继代培养，继代周期为30d，平均再生不定芽122个；外植体再生的不定芽扩繁1次转移至营养钵基质上生根和练苗，增殖系数达373，增殖培养基为MS + 6 BA 05mg/L+ NAA 02mg/L；生根培养基中IBA为20mg/L生根最好，生根率达9889%；揭开培养瓶盖驯化小植株容器苗，使之适应自然环境，驯化成活率达925%。

Micropropagation Techniques of Hybrid Aspen Clone 717 1B4

In order to maintain the maternal excellent features in offspring and refrain from difficult rooting in vegetative propagation of hybrid aspen, established a juvenility and container rooting hardening micropropagation system in the present work. The result showed that explants taken from the sprouting between root shoot junctions of maternal plants, sterilized by soaked 10% effective chlorine of sodium dichloroisocyanurate (NaDCC) solution for 25min, cultured on MS medium, 7591% of them regenerated juvenile shoots. The explants regenerated more than 3 juvenile shoots were selected and sub cultured on the same fresh medium in each 30 days, each explant produced 122 shoots on average. The shoots from explants multiplicated one time before did container rooting and hardening. The shoots increased 373 times on MS medium containing 6 BA 05mg/L and NAA 02mg/L, respectively. The rooting rate reached the highest at 9889% when IBA was 20mg/L in rooting medium. The container plantlets hardened in reversion and acclimatization by shifting and opening gradually the tissue culture bottles. The container rooted aspen plants survived at 925% in the acclimatization stage.