| 胎鼠额叶皮质神经干细胞的分离培养与分化 |
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| 引用本文： | 刘兵,欧微,刘洪涛.胎鼠额叶皮质神经干细胞的分离培养与分化[J].长江大学学报,2009,6(3):8-10,77. |
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| 作者姓名： | 刘兵 欧微 刘洪涛 |
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| 作者单位： | 长江大学医学院;长江大学附属第一医院荆州市第一人民医院; |
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| 摘 要： | 目的:探索从胚胎小鼠额叶皮质分离培养神经干细胞的方法。方法:无菌条件下分离孕13～15d小鼠胚胎额叶皮质脑组织,经消化及机械吹打成单细胞后接种于含有B27、bFGF的DMEM/F12培养基,培养扩增;倒置显微镜观察比较生长状况;机械分离法传代;10%血清接种分化;免疫细胞化学方法染色鉴定神经干细胞及其子代细胞的分化方向,结果:培养的部分细胞体外分裂增殖,同时表达神经干细胞特异性抗原nestin,并向神经细胞和胶质细胞分化。结论:小鼠胚脑存在具有多分化潜能的神经干细胞,它们能在体外稳定培养、传代和分化,为细胞替代治疗提供了理想的细胞来源。
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| 关 键 词： | 神经干细胞 培养 分化 胎鼠 |
Isolation Culture and Differentiation of Neural Stem Cells from the Cerebral Cortex of Frontal Iobe in Embryonic Mice |
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| Liu Bing,Ou Wei Liu Hong-tao.Isolation Culture and Differentiation of Neural Stem Cells from the Cerebral Cortex of Frontal Iobe in Embryonic Mice[J].Journal of Yangtze University,2009,6(3):8-10,77. |
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| Authors: | Liu Bing Ou Wei Liu Hong-tao |
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| Institution: | Medical School of Yangtze University;Jingzhou 434023;China;the First Hospital of Yangtze University;Jingzhou 434000;China;Medical School of Yangtze University;China |
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| Abstract: | Objective:To explore the culture methods of neural stem cells f rom cerebral cortex of frontal lobe in embryonic mice and to Understand the NSCs biological property.Methods:cortex of frontal lobe in embryonic mice(E13-15d) were dissociated and single cell suspensions were achieved in sterile condtions.Then the cells were cultured in DMEM/F12 supplemented with bFGF a nd B27.Immunocylochemical techniques were used to odentify stem cells and their progeny;cell growth was observed by Inverted microscope;then pa... |
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| Keywords: | Neural stem cells Culture Differentiation Embryonic mice |
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