| 八角莲的愈伤组织和组培根及野生根状茎的鬼臼毒素含量比较研究 |
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| 引用本文： | 潘琦,陈绍瑗,姜维梅,傅承新.八角莲的愈伤组织和组培根及野生根状茎的鬼臼毒素含量比较研究[J].浙江大学学报(农业与生命科学版),2006,32(1):56-59. |
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| 作者姓名： | 潘琦 陈绍瑗 姜维梅 傅承新 |
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| 作者单位： | 1. 浙江大学,生命科学学院,植物系统进化与生物多样性实验室,浙江,杭州,310029
2. 香港理工大学应用生物与化学系,香港 |
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| 摘 要： | 比较研究了峨眉山野生八角莲根、组培愈伤组织及其诱导根的鬼臼毒素含量,为八角莲的快速繁殖和利用生物技术生产鬼臼毒素提供科学依据.高效液相色谱技术(HPLC)显示组织培养的八角莲愈伤组织和诱导根中均含有鬼臼毒素,分别为0.117%和0.148%,野生品的根中其含量为0.600%.通过组织培养可在不破坏自然资源的前提下快速生产鬼臼毒素,从而为开发鬼臼毒素类抗癌药物提供大量原料.
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| 关 键 词： | 鬼臼毒素 八角莲 HPLC 组织培养 抗肿瘤作用 |
| 文章编号： | 1008-9209（2006）01-0056-04 |
| 收稿时间： | 2004-05-10 |
| 修稿时间： | 2004-05-10 |
Study on the podophyllotoxin in the Dysosma versipellis (Hance) M. Cheng and its Callus' Materials |
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| PAN Qi,CHEN Shao-yuan,JIANG Wei-mei,Gilbert Y S Chan,FU Cheng-xin.Study on the podophyllotoxin in the Dysosma versipellis (Hance) M. Cheng and its Callus'''' Materials[J].Journal of Zhejiang University(Agriculture & Life Sciences),2006,32(1):56-59. |
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| Authors: | PAN Qi CHEN Shao-yuan JIANG Wei-mei Gilbert Y S Chan FU Cheng-xin |
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| Institution: | 1. Lab of Plant Systematic Evolution and Biodiversity, College of Life Sciences, Zhejiang University, Hangzhou 310029, China; 2. Department of Applied Biology and Chemical Technology, Hongkong Polytechnic University, Hongkong, China |
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| Abstract: | In this study,HPLC was used to detect the podophyllotoxin content in three parts of Dysosma versipellis(Hance).The content was 0.60% in the wild root,while in the calli and the induced root from this calli,they were 0.117% and 0.148%,respectively.The results suggest that it is possible to produce podophyllotoxin quickly by tissue culture,and provide much raw material to antitumor drugs with little damage to the natural resources. |
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| Keywords: | HPLC |
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